ArticleOncogene2000
Genetic screens in mammalian cells by enhanced retroviral mutagens.
Article in Oncogene, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 42 citations in OpenAlex.
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- CCPG1 Is a Non-canonical Autophagy Cargo Receptor Essential for ER-Phagy and Pancreatic ER Proteostasis.Developmental cell · 2018Article
- Genome-wide identification of histone H2A and histone variant H2A.Z-interacting proteins by bPPI-seq.Cell research · 2017Article
- Kinetics of recruitment and allosteric activation of ARHGEF25 isoforms by the heterotrimeric G-protein Gαq.Scientific reports · 2016Article
- Plasma membrane restricted RhoGEF activity is sufficient for RhoA-mediated actin polymerization.Scientific reports · 2015Article
- Regulation of Latent Membrane Protein 1 Signaling through Interaction with Cytoskeletal Proteins.Journal of virology · 2015Article
- Signaling efficiency of Gαq through its effectors p63RhoGEF and GEFT depends on their subcellular location.Scientific reports · 2013Article
- Identification of transmembrane protein 134 as a novel LMP1-binding protein by using bimolecular fluorescence complementation and an enhanced retroviral mutagen.Journal of virology · 2012Article
- Characterization of the latent membrane protein 1 signaling complex of Epstein-Barr virus in the membrane of mammalian cells with bimolecular fluorescence complementation.Virology journal · 2011Article
- Use of forward genetics to discover novel regulators of NF-kappaB.Cold Spring Harbor perspectives in biology · 2010Review
- Sleeping beauty-mediated somatic mutagenesis implicates CSF1 in the formation of high-grade astrocytomas.Cancer research · 2010Article
- The onconeural antigen cdr2 is a novel APC/C target that acts in mitosis to regulate c-myc target genes in mammalian tumor cells.PloS one · 2010Article
- Gene trapping identifies chloride channel 4 as a novel inducer of colon cancer cell migration, invasion and metastases.British journal of cancer · 2010Article
- CARD9 facilitates microbe-elicited production of reactive oxygen species by regulating the LyGDI-Rac1 complex.Nature immunology · 2009Article
- Tumor antigen LRRC15 impedes adenoviral infection: implications for virus-based cancer therapy.Journal of virology · 2008Article
- Galphaq directly activates p63RhoGEF and Trio via a conserved extension of the Dbl homology-associated pleckstrin homology domain.The Journal of biological chemistry · 2007Article
- Genetic screening reveals an essential role of p27kip1 in restriction of breast cancer progression.Cancer research · 2007Article
- A retrovirus-based protein complementation assay screen reveals functional AKT1-binding partners.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetic approaches such as retrovirus-mediated mutagenesis and cDNA expression libraries have contributed greatly to our understanding of signal transduction in mammalian cells. However, previously described methods for retroviral insertional mutagenesis are hindered by low mutagenesis rates and difficulties in cloning mutated genes. cDNA expression library methods are usually cell-type dependent and bias towards abundant and short messages. With the near completion of the genome projects, alternative genetic methods are needed where large numbers of genes can be more easily isolated and biochemically studied. We have developed a novel retrovirus-mediated genetic screening method in cultured cells. To achieve efficient and regulated mutagenesis, we constructed Enhanced Retroviral Mutagen (ERM) vectors that contained several engineered sequences (e.g., an ERM Tag and a splice donor) controlled by a tetracycline-responsive promoter. Endogenous genes can thus be randomly activated and tagged in a conditional system. NIH3T3 cells were used to screen for focus-forming genes using the ERM strategy. We showed that these added sequences increased the screening efficiency by >10-fold, and allowed more direct identification of the genes targeted. Sequence analysis of approximately 10% of the >600 focus clones recovered revealed both known oncogenes and novel factors such as protein kinases and GTP/GDP exchange proteins. The ERM strategy should help to facilitate large-scale gene identification in diverse pathways and integrate both genetic (with the completion of the genome projects) and functional information more readily.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.