ArticleJournal of biomolecular NMR2000
An approach for high-throughput structure determination of proteins by NMR spectroscopy.
Article in Journal of biomolecular NMR, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Global protein dynamics as communication sensors in peptide synthetase domains.Science advances · 2022Article
- Increased resolution of aromatic cross peaks using alternate 13C labeling and TROSY.Journal of biomolecular NMR · 2015Article
- Dcp1 links coactivators of mRNA decapping to Dcp2 by proline recognition.RNA (New York, N.Y.) · 2011Article
- Intermolecular interactions in a 44 kDa interferon-receptor complex detected by asymmetric reverse-protonation and two-dimensional NOESY.Biochemistry · 2010Article
- An extended structure of the APOBEC3G catalytic domain suggests a unique holoenzyme model.Journal of molecular biology · 2009Article
- Substrate binding to cytochromes P450.Analytical and bioanalytical chemistry · 2008Review
- Perspectives on NMR in drug discovery: a technique comes of age.Nature reviews. Drug discovery · 2008Review
- Investigation of the utility of selective methyl protonation for determination of membrane protein structures.Journal of biomolecular NMR · 2008Article
- Article
- Identification of individual protein-ligand NOEs in the limit of intermediate exchange.Journal of biomolecular NMR · 2006Article
- NMR distinction of single- and multiple-mode binding of small-molecule protein ligands.Journal of the American Chemical Society · 2006Article
- Using codon optimization, chaperone co-expression, and rational mutagenesis for production and NMR assignments of human eIF2 alpha.Journal of biomolecular NMR · 2004Article
- Solution structure and function of an essential CMP kinase of Streptococcus pneumoniae.Protein science : a publication of the Protein Society · 2003Article
- A novel method for the biosynthesis of deuterated proteins with selective protonation at the aromatic rings of Phe, Tyr and Trp.Journal of biomolecular NMR · 2003Article
- Automated protein fold determination using a minimal NMR constraint strategy.Protein science : a publication of the Protein Society · 2003Article
- A sensitive and robust method for obtaining intermolecular NOEs between side chains in large protein complexes.Journal of biomolecular NMR · 2003Article
- Mapping the binding interface between human eukaryotic initiation factors 1A and 5B: a new interaction between old partners.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Prediction algorithm for amino acid types with their secondary structure in proteins (PLATON) using chemical shifts.Journal of biomolecular NMR · 2003Article
- Solution structure of a Bcl-2 homolog from Kaposi sarcoma virus.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Exact solutions for chemical bond orientations from residual dipolar couplings.Journal of biomolecular NMR · 2002Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An approach is described for rapidly determining protein structures by NMR that utilizes proteins containing 13C-methyl labeled Val, Leu, and Ile (delta1) and protonated Phe and Tyr in a deuterated background. Using this strategy, the key NOEs that define the hydrophobic core and overall fold of the protein are easily obtained. NMR data are acquired using cryogenic probe technology which markedly reduces the spectrometer time needed for data acquisition. The approach is demonstrated by determining the overall fold of the antiapoptotic protein, Bcl-xL, from data collected in only 4 days. Refinement of the Bcl-xL structure to a backbone rmsd of 0.95 A was accomplished with data collected in an additional 3 days. A distance analysis of 180 different proteins and structure calculations using simulated data suggests that our method will allow the global folds of a wide variety of proteins to be determined.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.