ArticleRNA (New York, N.Y.)2000
Dynamics and three-dimensional localization of ribosomal RNA within the nucleolus.
Article in RNA (New York, N.Y.), 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Intrinsically disordered regions stimulate concentration of small nucleolar ribonucleoproteins and formation of Cajal bodies and nucleoli.Genes & development · 2026Article
- Article
- Article
- Viscoelasticity and advective flow of RNA underlies nucleolar form and function.Molecular cell · 2023Article
- Review
- The nucleolus as a multiphase liquid condensate.Nature reviews. Molecular cell biology · 2021Review
- Structure and epigenetics of nucleoli in comparison with non-nucleolar compartments.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2010Review
- Localization of Nopp140 within mammalian cells during interphase and mitosis.Histochemistry and cell biology · 2009Article
- Chromatin tethering effects of hNopp140 are involved in the spatial organization of nucleolus and the rRNA gene transcription.Journal of biomedical science · 2008Article
- Intranucleolar sites of ribosome biogenesis defined by the localization of early binding ribosomal proteins.The Journal of cell biology · 2007Article
- Different domains control the localization and mobility of LIKE HETEROCHROMATIN PROTEIN1 in Arabidopsis nuclei.The Plant cell · 2006Article
- The moving parts of the nucleolus.Histochemistry and cell biology · 2005Review
- NO66, a highly conserved dual location protein in the nucleolus and in a special type of synchronously replicating chromatin.Molecular biology of the cell · 2004Article
- Diffusion-based transport of nascent ribosomes in the nucleus.Molecular biology of the cell · 2003Article
- Modulation of RNA editing by functional nucleolar sequestration of ADAR2.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Mammalian and yeast U3 snoRNPs are matured in specific and related nuclear compartments.The EMBO journal · 2002Article
- Ribosomal genes in focus: new transcripts label the dense fibrillar components and form clusters indicative of "Christmas trees" in situ.The Journal of cell biology · 2002Article
- Building an efficient factory: where is pre-rRNA synthesized in the nucleolus?The Journal of cell biology · 2002Review
- Conventional and nonconventional roles of the nucleolus.International review of cytology · 2002Review
- A novel karyoskeletal protein: characterization of protein NO145, the major component of nucleolar cortical skeleton in Xenopus oocytes.Molecular biology of the cell · 2001Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although rRNA synthesis, maturation, and assembly into preribosomal particles occur within the nucleolus, the route taken by pre-rRNAs from their synthetic sites toward the cytoplasm remains largely unexplored. Here, we employed a nondestructive method for the incorporation of BrUTP into the RNA of living cells. By using pulse-chase experiments, three-dimensional image reconstructions of confocal optical sections, and electron microscopy analysis of ultrathin sections, we were able to describe topological and spatial dynamics of rRNAs within the nucleolus. We identified the precise location and the volumic organization of four typical subdomains, in which rRNAs are successively moving towards the nucleolar periphery during their synthesis and processing steps. The incorporation of BrUTP takes place simultaneously within several tiny spheres, centered on the fibrillar centers. Then, the structures containing the newly synthesized RNAs enlarge and appear as compact ringlets disposed around the fibrillar centers. Later, they form hollow spheres surrounding the latter components and begin to fuse together. Finally, these structures widen and form large rings reaching the limits of the nucleoli. These results clearly show that the transport of pre-rRNAs within the nucleolus does not occur randomly, but appears as a radial flow starting from the fibrillar centers that form concentric rings, which finally fuse together as they progress toward the nucleolar periphery.
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