ArticleRNA (New York, N.Y.)2000
Nucleotides of 18S rRNA surrounding mRNA codons at the human ribosomal A, P, and E sites: a crosslinking study with mRNA analogs carrying an aryl azide group at either the uracil or the guanine residue.
Article in RNA (New York, N.Y.), 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 52 citations in OpenAlex.
- Structural Insights into the Mammalian Late-Stage Initiation Complexes.Cell reports · 2020Article
- A guide to naming human non-coding RNA genes.The EMBO journal · 2020Review
- Integrative rDNAomics-Importance of the Oldest Repetitive Fraction of the Eukaryote Genome.Genes · 2019Review
- Structural rearrangements in mRNA upon its binding to human 80S ribosomes revealed by EPR spectroscopy.Nucleic acids research · 2018Article
- The Expansion Segments of 28S Ribosomal RNA Extensively Match Human Messenger RNAs.Frontiers in genetics · 2018Article
- Doubly Spin-Labeled RNA as an EPR Reporter for Studying Multicomponent Supramolecular Assemblies.Biophysical journal · 2015Article
- Interaction of tRNA with eukaryotic ribosome.International journal of molecular sciences · 2015Review
- Article
- Structures of yeast 80S ribosome-tRNA complexes in the rotated and nonrotated conformations.Structure (London, England : 1993) · 2014Article
- A central fragment of ribosomal protein S26 containing the eukaryote-specific motif YxxPKxYxK is a key component of the ribosomal binding site of mRNA region 5' of the E site codon.Nucleic acids research · 2012Article
- A multifunctional bioconjugate module for versatile photoaffinity labeling and click chemistry of RNA.Nucleic acids research · 2011Article
- The human IGF1R IRES likely operates through a Shine-Dalgarno-like interaction with the G961 loop (E-site) of the 18S rRNA and is kinetically modulated by a naturally polymorphic polyU loop.Journal of cellular biochemistry · 2010Article
- Positioning of subdomain IIId and apical loop of domain II of the hepatitis C IRES on the human 40S ribosome.Nucleic acids research · 2009Article
- Identification of protein-coding sequences using the hybridization of 18S rRNA and mRNA during translation.Nucleic acids research · 2009Article
- Ribosomal position and contacts of mRNA in eukaryotic translation initiation complexes.The EMBO journal · 2008Article
- Specific functional interactions of nucleotides at key -3 and +4 positions flanking the initiation codon with components of the mammalian 48S translation initiation complex.Genes & development · 2006Article
- RNA nanotechnology: engineering, assembly and applications in detection, gene delivery and therapy.Journal of nanoscience and nanotechnology · 2005Review
- The central part of the 5.8 S rRNA is differently arranged in programmed and free human ribosomes.The Biochemical journal · 2005Article
- Investigation of Mg2+- and temperature-dependent folding of the hairpin ribozyme by photo-crosslinking: effects of photo-crosslinker tether length and chemistry.Nucleic acids research · 2005Article
- Targeting the A site RNA of the Escherichia coli ribosomal 30 S subunit by 2'-O-methyl oligoribonucleotides: a quantitative equilibrium dialysis binding assay and differential effects of aminoglycoside antibiotics.The Biochemical journal · 2004Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 18S rRNA environment of the mRNA at the decoding site of human 80S ribosomes has been studied by cross-linking with derivatives of hexaribonucleotide UUUGUU (comprising Phe and Val codons) that carried a perfluorophenylazide group either at the N7 atom of the guanine or at the C5 atom of the 5'-terminal uracil residue. The location of the codons on the ribosome at A, P, or E sites has been adjusted by the cognate tRNAs. Three types of complexes have been obtained for each type derivative, namely, (1) codon UUU and Phe-tRNAPhe at the P site (codon GUU at the A site), (2) codon UUU and tRNAPhe at the P site and PheVal-tRNAVal at the A site, and (3) codon GUU and Val-tRNAVal at the P site (codon UUU at the E site). This allowed the placement of modified nucleotides of the mRNA analog at positions -3, +1, or +4 on the ribosome. Mild UV irradiation resulted in tRNA-dependent crosslinking of the mRNA analogs to the 18S rRNA. Nucleotide G961 crosslinked to mRNA position -3, nucleotide G1207 to position +1, and A1823 together with A1824 to position +4. All of these nucleotides are located in the most strongly conserved regions of the small subunit RNA structure, and correspond to nucleotides G693, G926, G1491, and A1492 of bacterial 16S rRNA. Three of them (with the exception of G1491) had been found earlier at the 70S ribosomal decoding site. The similarities and differences between the 16S and 18S rRNA decoding sites are discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.