ArticleAnalytical biochemistry2001
On the possibility of long-wavelength long-lifetime high-quantum-yield luminophores.
Article in Analytical biochemistry, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed.
- Recent Advances in the Synthesis and Optical Applications of Acridine-based Hybrid Fluorescent Dyes.Journal of fluorescence · 2025Review
- Direct Observation of Triplet-Triplet Energy Transfer in DNA between Energy Donor and Acceptor C‑Nucleotides.JACS Au · 2025Article
- Quantitative and comparative assessment of dyes and protocols for rapid ex vivo microscopy of fresh tissues.Scientific reports · 2024Article
- Ruthenium(II) Polypyridyl Complexes as FRET Donors: Structure- and Sequence-Selective DNA-Binding and Anticancer Properties.Journal of the American Chemical Society · 2023Article
- Interaction of Flavonoids from Woodwardia unigemmata with Bovine Serum Albumin (BSA): Application of Spectroscopic Techniques and Molecular Modeling Methods.Molecules (Basel, Switzerland) · 2017Article
- Bichromophoric dyes for wavelength shifting of dye-protein fluoromodules.Organic & biomolecular chemistry · 2015Article
- Dynamics of tRNAtyr probed with long-lifetime metal-ligand complexes.Journal of fluorescence · 2011Article
- Long wavelength fluorescence lifetime standards for front-face fluorometry.Journal of fluorescence · 2010Article
- Dopamine 5 receptor mediates Ang II type 1 receptor degradation via a ubiquitin-proteasome pathway in mice and human cells.The Journal of clinical investigation · 2008Article
- Electrical detection of TATA binding protein at DNA-modified microelectrodes.Journal of the American Chemical Society · 2008Article
- FRET studies of the interaction of dimeric cyanine dyes with DNA.Journal of fluorescence · 2004Article
- Real-time background suppression during frequency domain lifetime measurements.Analytical biochemistry · 2002Article
- Dynamics of supercoiled and relaxed pTZ18U plasmids probed with a long-lifetime metal-ligand complex.Journal of biochemistry and molecular biology · 2002Article
- DNA dynamics: a fluorescence resonance energy transfer study using a long-lifetime metal-ligand complex.Archives of pharmacal research · 2002Article
- Enhanced Emission Induced by FRET from a Long-Lifetime, Low Quantum Yield Donor to a Long-Wavelength, High Quantum Yield Acceptor.Journal of fluorescence · 2002Article
- Lateral diffusion coefficients in membranes measured by resonance energy transfer and a new algorithm for diffusion in two dimensions.Biophysical journal · 2002Article
- Molecular immunolabeling with recombinant single-chain variable fragment (scFv) antibodies designed with metal-binding domains.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Fluorescence Resonance Energy Transfer in Calf Thymus DNA from a Long-Lifetime Metal-Ligand Complex to Nile Blue.Journal of biochemistry and molecular biology · 2001Article
- Rab4 and Rab11 coordinately regulate the recycling of angiotensin II type I receptor as demonstrated by fluorescence resonance energy transfer microscopy.Journal of biomedical opticsArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
We describe an approach to creating a new class of luminophores which display both long wavelength emissions exceeding 600 nm and long lifetimes. These luminophores are based on resonance energy transfer (RET) from a long lifetime donor to a short lifetime but long wavelength acceptor. We demonstrated the possibility of obtaining these desirable spectral properties using donors and acceptors noncovalently bound to DNA. The donor was a ruthenium (Ru) metal-ligand complex in which one of the diimine ligands intercalated into double-helix DNA. The acceptors were either nile blue, TOTO-3, or TO-PRO-3. Upon binding of the acceptor to donor-labeled DNA, we found that the acceptor quantum yield was remarkably enhanced so that the wavelength-integrated intensities of the donor and acceptor bound to DNA were many-fold greater than the intensity of the donor and acceptor alone when separately bound to DNA. The origin of this effect is efficient energy transfer from the donor. Under these conditions the effective overall quantum yield approaches that of the acceptor. Importantly, the increased quantum yield can be obtained while maintaining usefully long apparent acceptor lifetimes of 30 to 80 ns. The effect of an increased quantum yield from a low quantum yield donor may find use in assays to detect macromolecular binding interactions. These results suggest the synthesis of covalently linked donor-acceptor pairs with the desirable spectral properties of long wavelength emission, high quantum yield, and moderately long lifetimes for gated detection.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.