ArticleNucleic acids research2001
Inducible expression of exogenous genes in Dictyostelium discoideum using the ribonucleotide reductase promoter.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 19 citations in OpenAlex.
- Identification and characterization of alternative promoters, transcripts and protein isoforms of zebrafish R2 gene.PloS one · 2011Article
- Article
- Formation of Hirano bodies after inducible expression of a modified form of an actin-cross-linking protein.Eukaryotic cell · 2009Article
- Adenylyl cyclase G is activated by an intramolecular osmosensor.Molecular biology of the cell · 2004Article
- Involvement of a novel gene, zyg1, in zygote formation of Dictyostelium mucoroides.Journal of muscle research and cell motility · 2002Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report here the development of a regulated gene expression system for Dictyostelium discoideum based on the DNA-damage inducibility of the rnrB gene. rnrB, which codes for the small subunit of the enzyme ribonucleotide reductase, responds to DNA-damaging agents at all stages of the D.discoideum life cycle. Doses that have little effect on development have previously been shown to increase the level of the rnrB transcript by up to 15-fold. Here we show that all elements necessary for DNA-damage induction are contained in a 450 bp promoter fragment. We used a fusion of the rnrB promoter with the gene encoding GFP to demonstrate an up to 10-fold induction at the RNA level, which appears in all aspects similar to induction of the endogenous rnrB transcript. Using a fusion with the lacZ gene we observed an up to 7-fold induction at the protein level. These results indicate that the rnrB promoter can be used to regulate the expression of specific genes in D.discoideum. This controllable gene expression system provides the following new characteristics: the induction is rapid, taking place in the order of minutes, and the promoter is responsive at all stages of the D.discoideum life cycle.
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Registered trials
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