ArticleNucleic acids research2001
RNA-templated DNA ligation for transcript analysis.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 99 citations in OpenAlex.
- Genetic encoding and expression of RNA origami cytoskeletons in synthetic cells.Nature nanotechnology · 2025Article
- Quantitative and spatially resolved detection of multiplexed microRNA from plant tissue via hybridization to hydrogel-bound DNA probes in nanoliter well arrays.Microsystems & nanoengineering · 2024Article
- Point-of-Care Tests for HIV Drug Resistance Monitoring: Advances and Potentials.Pathogens (Basel, Switzerland) · 2022Article
- Rolling Circle Amplification in Integrated Microsystems: An Uncut Gem toward Massively Multiplexed Pathogen Diagnostics and Genotyping.Accounts of chemical research · 2021Review
- Highly multiplexed oligonucleotide probe-ligation testing enables efficient extraction-free SARS-CoV-2 detection and viral genotyping.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2021Article
- Pyrococcus furiosus Argonaute coupled with modified ligase chain reaction for detection of SARS-CoV-2 and HPV.Talanta · 2021Article
- A Ligation/Recombinase Polymerase Amplification Assay for Rapid Detection of SARS-CoV-2.Frontiers in cellular and infection microbiology · 2021Article
- Chimeric padlock and iLock probes for increased efficiency of targeted RNA detection.RNA (New York, N.Y.) · 2019Article
- RNase H-assisted RNA-primed rolling circle amplification for targeted RNA sequence detection.Scientific reports · 2018Article
- Fidelity of RNA templated end-joining by chlorella virus DNA ligase and a novel iLock assay with improved direct RNA detection accuracy.Nucleic acids research · 2017Article
- Efficient in situ detection of mRNAs using the Chlorella virus DNA ligase for padlock probe ligation.RNA (New York, N.Y.) · 2017Article
- Archaeal RNA ligase from thermoccocus kodakarensis for template dependent ligation.RNA biology · 2017Article
- One-Step Ligation on RNA Amplification for the Detection of Point Mutations.The Journal of molecular diagnostics : JMD · 2015Article
- Article
- Efficient DNA ligation in DNA-RNA hybrid helices by Chlorella virus DNA ligase.Nucleic acids research · 2014Article
- Amplified microRNA detection by templated chemistry.Nucleic acids research · 2012Article
- Sensitive RNA detection by combining three-way junction formation and primer generation-rolling circle amplification.Nucleic acids research · 2012Article
- Template-directed ligation of tethered mononucleotides by t4 DNA ligase for kinase ribozyme selection.PloS one · 2010Article
- Direct detection of RNA in vitro and in situ by target-primed RCA: The impact of E. coli RNase III on the detection efficiency of RNA sequences distanced far from the 3'-end.RNA (New York, N.Y.) · 2010Article
- Novel application of Phi29 DNA polymerase: RNA detection and analysis in vitro and in situ by target RNA-primed RCA.RNA (New York, N.Y.) · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ligase-mediated gene detection has proven valuable for detection and precise distinction of DNA sequence variants. We have recently shown that T4 DNA ligase can also be used to distinguish single nucleotide variants of RNA sequences. Here we describe parameters that influence RNA-templated DNA ligation by T4 DNA ligase. The reaction proceeds much more slowly, requiring more enzyme, compared to ligation of the same oligonucleotides hybridized to the corresponding DNA sequence. The reaction is inhibited at high concentrations of ATP and NaCl and both magnesium and manganese ions can support the reaction. We define reaction conditions where 80% of RNA target molecules can template a diagnostic ligation reaction. Ligase-mediated RNA detection should provide a useful mechanism for sensitive and accurate detection and distinction of RNA sequence variants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.