ArticleNucleic acids research2001
Antagonistic action of Six3 and Prox1 at the gamma-crystallin promoter.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 69 citations in OpenAlex.
- Hippo signaling regulates dorsoventral patterning iniScience · 2026Article
- Mouse models for microphthalmia, anophthalmia and cataracts.Human genetics · 2019Review
- Signaling and Gene Regulatory Networks in Mammalian Lens Development.Trends in genetics : TIG · 2017Review
- An HDAC3-PROX1 corepressor module acts on HNF4α to control hepatic triglycerides.Nature communications · 2017Article
- Prox1 and fibroblast growth factor receptors form a novel regulatory loop controlling lens fiber differentiation and gene expression.Development (Cambridge, England) · 2016Article
- Patterns of gene expression in microarrays and expressed sequence tags from normal and cataractous lenses.Human genomics · 2012Article
- The lens in focus: a comparison of lens development in Drosophila and vertebrates.Molecular genetics and genomics : MGG · 2011Review
- Mutations in the human SIX3 gene in holoprosencephaly are loss of function.Human molecular genetics · 2008Article
- Dual roles for Prox1 in the regulation of the chicken betaB1-crystallin promoter.Investigative ophthalmology & visual science · 2008Article
- PCNA interacts with Prox1 and represses its transcriptional activity.Molecular vision · 2008Article
- Genetic and epigenetic mechanisms of gene regulation during lens development.Progress in retinal and eye research · 2007Review
- Expression of Prox1 during mouse cochlear development.The Journal of comparative neurology · 2006Article
- Prox1 promotes lineage-specific expression of fibroblast growth factor (FGF) receptor-3 in lymphatic endothelium: a role for FGF signaling in lymphangiogenesis.Molecular biology of the cell · 2006Article
- Fibroblast growth factor receptor 1 (Fgfr1) is not essential for lens fiber differentiation in mice.Molecular vision · 2006Article
- Tissue-specific regulation of the mouse alphaA-crystallin gene in lens via recruitment of Pax6 and c-Maf to its promoter.Journal of molecular biology · 2005Article
- HSF4 is required for normal cell growth and differentiation during mouse lens development.The EMBO journal · 2004Article
- Regulation of gene expression by Pax6 in ocular cells: a case of tissue-preferred expression of crystallins in lens.The International journal of developmental biology · 2004Review
- Prox1 is a novel coregulator of Ff1b and is involved in the embryonic development of the zebra fish interrenal primordium.Molecular and cellular biology · 2003Article
- Multiple developmental defects derived from impaired recruitment of ASC-2 to nuclear receptors in mice: implication for posterior lenticonus with cataract.Molecular and cellular biology · 2002Article
- Mutually regulated expression of Pax6 and Six3 and its implications for the Pax6 haploinsufficient lens phenotype.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Gamma-crystallin genes are specifically expressed in the eye lens. Their promoters constitute excellent models to analyse tissue-specific gene expression. We investigated murine CRYGE/f promoters of different length in lens epithelial cell lines. The most active fragment extends from position -219 to +37. Computer analysis predicts homeodomain and paired-domain binding sites for all rodent CRYGD/e/f core promoters. As examples, we analysed the effects of Prox1 and Six3, which are considered important transcription factors involved in lens development. Because of endogenous Prox1 expression in N/N1003A cells, a weak stimulation of CRYGE/f promoter activity was found for PROX1. In contrast, PROX1 stimulated the CRYGF promoter 10-fold in CD5A cells without endogenous PROX1. In both cell lines Six3 repressed the CRYGF promoter to 10% of its basal activity. Our cell transfection experiments indicated that CRYG expression increases as Six3 expression decreases. Prox1 and Six3 act antagonistically on regulation of the CRYGD/e/f promoters. Functional assays using randomly mutated gammaF-crystallin promoter fragments define a Six3-responsive element between -101 and -123 and a Prox1-responsive element between -151 and -174. Since Prox1 and Six3 are present at the beginning of lens development, expression of CRYGD/e/f is predicted to remain low at this time. It increases as Six3 expression decreases during ongoing lens development.
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