ArticleNucleic acids research2001
Characterization of Schizosaccharomyces pombe RNA triphosphatase.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- Enzymatic Assays to Explore Viral mRNA Capping Machinery.Chembiochem : a European journal of chemical biology · 2021Review
- Article
- Structural Determinants for Substrate Binding and Catalysis in Triphosphate Tunnel Metalloenzymes.The Journal of biological chemistry · 2015Article
- Fission yeast RNA triphosphatase reads an Spt5 CTD code.RNA (New York, N.Y.) · 2015Article
- A novel role for Cet1p mRNA 5'-triphosphatase in promoter proximal accumulation of RNA polymerase II in Saccharomyces cerevisiase.Genetics · 2014Article
- Deciphering the RNA polymerase II CTD code in fission yeast.Molecular cell · 2011Article
- Nucleotide analogs and molecular modeling studies reveal key interactions involved in substrate recognition by the yeast RNA triphosphatase.Nucleic acids research · 2009Article
- Polyphosphatase activity of CthTTM, a bacterial triphosphate tunnel metalloenzyme.The Journal of biological chemistry · 2008Article
- Inhibition of a metal-dependent viral RNA triphosphatase by decavanadate.The Biochemical journal · 2006Article
- Structure-function analysis of Plasmodium RNA triphosphatase and description of a triphosphate tunnel metalloenzyme superfamily that includes Cet1-like RNA triphosphatases and CYTH proteins.RNA (New York, N.Y.) · 2006Article
- What messenger RNA capping tells us about eukaryotic evolution.Nature reviews. Molecular cell biology · 2002Review
- Divergent subunit interactions among fungal mRNA 5'-capping machineries.Eukaryotic cell · 2002Article
- A yeast-like mRNA capping apparatus in Plasmodium falciparum.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- RNA triphosphatase is essential in Schizosaccharomyces pombe and Candida albicans.BMC microbiology · 2001Article
- mRNA:guanine-N7 cap methyltransferases: identification of novel members of the family, evolutionary analysis, homology modeling, and analysis of sequence-structure-function relationships.BMC bioinformatics · 2001Article
- The RNA capping machinery as an anti-infective target.Wiley interdisciplinary reviews. RNAReview
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
RNA triphosphatase catalyzes the first step in mRNA cap formation which entails the cleavage of the beta-gamma phosphoanhydride bond of triphosphate-terminated RNA to yield a diphosphate end that is then capped with GMP by RNA guanylyltransferase. Here we characterize a 303 amino acid RNA triphosphatase (Pct1p) encoded by the fission yeast SCHIZOSACCHAROMYCES: pombe. Pct1p hydrolyzes the gamma phosphate of triphosphate-terminated poly(A) in the presence of magnesium. Pct1p also hydrolyzes ATP to ADP and P(i) in the presence of manganese or cobalt (K(m) = 19 microM ATP; k(cat) = 67 s(-1)). Hydrolysis of 1 mM ATP is inhibited with increasing potency by inorganic phosphate (I(0.5) = 1 mM), pyrophosphate (I(0.5) = 0.4 mM) and tripolyphosphate (I(0.5) = 30 microM). Velocity sedimentation indicates that Pct1p is a homodimer. Pct1p is biochemically and structurally similar to the catalytic domain of Saccharomyces cerevisiae RNA triphosphatase Cet1p. Mechanistic conservation between Pct1p and Cet1p is underscored by a mutational analysis of the putative metal-binding site of Pct1p. Pct1p is functional in vivo in S.cerevisiae in lieu of Cet1p, provided that it is coexpressed with the S.pombe guanylyltransferase. Pct1p and other yeast RNA triphosphatases are completely unrelated, mechanistically and structurally, to the metazoan RNA triphosphatases, suggesting an abrupt evolutionary divergence of the capping apparatus during the transition from fungal to metazoan species.
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