ArticleGenetics2001
Extragenic bypass suppressors of mutations in the essential gene BLD2 promote assembly of basal bodies with abnormal microtubules in Chlamydomonas reinhardtii.
Article in Genetics, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- Genome editing in the green alga Chlamydomonas: past, present practice and future prospects.The Plant journal : for cell and molecular biology · 2025Review
- Undocking of an extensive ciliary network induces proteostasis and cell fate switching resulting in severe primary ciliary dyskinesia.Science translational medicine · 2025Article
- Loss of an extensive ciliary connectome induces proteostasis and cell fate switching in a severe motile ciliopathy.bioRxiv : the preprint server for biology · 2024Article
- Microtubules grow by the addition of bent guanosine triphosphate tubulin to the tips of curved protofilaments.The Journal of cell biology · 2018Article
- Review
- Article
- The basal bodies of Chlamydomonas reinhardtii.Cilia · 2016Review
- Zeta-Tubulin Is a Member of a Conserved Tubulin Module and Is a Component of the Centriolar Basal Foot in Multiciliated Cells.Current biology : CB · 2015Article
- Conserved and divergent features of kinetochores and spindle microtubule ends from five species.The Journal of cell biology · 2013Article
- Article
- The simplest integrated multicellular organism unveiled.PloS one · 2013Article
- Whole-Genome Sequencing to Identify Mutants and Polymorphisms in Chlamydomonas reinhardtii.G3 (Bethesda, Md.) · 2012Article
- The daughter four-membered microtubule rootlet determines anterior-posterior positioning of the eyespot in Chlamydomonas reinhardtii.Cytoskeleton (Hoboken, N.J.) · 2011Article
- Article
- Molecular characterization of centriole assembly in ciliated epithelial cells.The Journal of cell biology · 2007Article
- Mutant kinesin-2 motor subunits increase chromosome loss.Molecular biology of the cell · 2005Article
- Bld10p, a novel protein essential for basal body assembly in Chlamydomonas: localization to the cartwheel, the first ninefold symmetrical structure appearing during assembly.The Journal of cell biology · 2004Article
- Three-dimensional organization of basal bodies from wild-type and delta-tubulin deletion strains of Chlamydomonas reinhardtii.Molecular biology of the cell · 2003Article
- Epsilon-tubulin is an essential component of the centriole.Molecular biology of the cell · 2002Article
- Functional role of epsilon-tubulin in the assembly of the centriolar microtubule scaffold.The Journal of cell biology · 2002Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
bld2-1 mutant Chlamydomonas reinhardtii strains assemble basal bodies with singlet microtubules; bld2-1 cells display flagellar assembly defects as well as positioning defects of the mitotic spindle and cleavage furrow. To further understand the role of the BLD2 gene, we have isolated three new bld2 alleles and three partially dominant extragenic suppressors, rgn1-1, rgn1-2, and rgn1-3. bld2 rgn1-1 strains have phenotypes intermediate between those of bld2 and wild-type strains with respect to flagellar number, microtubule rootlet organization, cleavage furrow positioning, and basal body structural phenotypes. Instead of the triplet microtubules of wild-type cells, bld2 rgn1-1 basal bodies have mixtures of no, singlet, doublet, and triplet microtubules. The bld2-4 allele was made by insertional mutagenesis and identified in a noncomplementation screen in a diploid strain. The bld2-4 allele has a lethal phenotype based on mitotic segregation in diploid strains and in haploid strains generated by meiotic recombination. The lethal phenotype in haploid strains is suppressed by rgn1-1; these suppressed strains have similar phenotypes to other bld2 rgn1-1 double mutants. It is likely that BLD2 is an essential gene that is needed for basal body assembly and function.
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