ArticleGenetics2001
The formamidase gene of Aspergillus nidulans: regulation by nitrogen metabolite repression and transcriptional interference by an overlapping upstream gene.
Article in Genetics, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 60 citations in OpenAlex.
- Prospects of formamide as nitrogen source in biotechnological production processes.Applied microbiology and biotechnology · 2024Review
- Paracoccidioides lutzii Infects Galleria mellonella Employing Formamidase as a Virulence Factor.PLoS neglected tropical diseases · 2024Article
- Proteomics ofJournal of fungi (Basel, Switzerland) · 2023Article
- Nitrogen Journey in Plants: From Uptake to Metabolism, Stress Response, and Microbe Interaction.Biomolecules · 2023Review
- Proteomic Response ofJournal of fungi (Basel, Switzerland) · 2022Article
- Article
- Co-option of an extracellular protease for transcriptional control of nutrient degradation in the fungus Aspergillus nidulans.Communications biology · 2021Article
- Nitrogen, Iron and Zinc Acquisition: Key Nutrients toJournal of fungi (Basel, Switzerland) · 2021Review
- Transcriptional and proteomic insights into phytotoxic activity of interspecific potato hybrids with low glycoalkaloid contents.BMC plant biology · 2021Article
- A Multifaceted Role of Tryptophan Metabolism and Indoleamine 2,3-Dioxygenase Activity inFrontiers in immunology · 2017Review
- Multiple nuclear localization signals mediate nuclear localization of the GATA transcription factor AreA.Eukaryotic cell · 2014Article
- Nitrogen regulation of fungal secondary metabolism in fungi.Frontiers in microbiology · 2014Review
- Characterization of an Nmr homolog that modulates GATA factor-mediated nitrogen metabolite repression in Cryptococcus neoformans.PloS one · 2012Article
- Biochemical and mutational studies of the Bacillus cereus CECT 5050T formamidase support the existence of a C-E-E-K tetrad in several members of the nitrilase superfamily.Applied and environmental microbiology · 2011Article
- Nitrogen metabolite repression of metabolism and virulence in the human fungal pathogen Cryptococcus neoformans.Genetics · 2011Article
- Recent advances in nitrogen regulation: a comparison between Saccharomyces cerevisiae and filamentous fungi.Eukaryotic cell · 2008Review
- Gene discovery and transcript analyses in the corn smut pathogen Ustilago maydis: expressed sequence tag and genome sequence comparison.BMC genomics · 2007Article
- Differential expression of Aspergillus nidulans ammonium permease genes is regulated by GATA transcription factor AreA.Eukaryotic cell · 2006Article
- L-selective amidase with extremely broad substrate specificity from Ochrobactrum anthropi NCIMB 40321.Applied and environmental microbiology · 2005Article
- Nuclear accumulation of the GATA factor AreA in response to complete nitrogen starvation by regulation of nuclear export.Eukaryotic cell · 2005Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to utilize formamide as a sole nitrogen source has been found in numerous fungi. We have cloned the fmdS gene encoding a formamidase from Aspergillus nidulans and found that it belongs to a highly conserved family of proteins separate from the major amidase families. The expression of fmdS is primarily regulated via AreA-mediated nitrogen metabolite repression and does not require the addition of exogenous inducer. Consistent with this, deletion analysis of the 5' region of fmdS has confirmed the presence of multiple AreA-binding sites containing a characteristic core GATA sequence. Under carbon starvation conditions the response to nitrogen starvation is eliminated, indicating that the lack of a carbon source may result in inactivation of AreA. Sequence analysis and isolation of cDNAs show that a gene of unknown function lies directly 5' of fmdS with its transcript overlapping the fmdS coding region. Disruption of the 5' gene and analysis of the effects of overexpression of this gene on fmdS expression has shown that expression of this upstream gene interferes with fmdS transcription, resulting in a strong dependence on AreA activation for expression. Therefore the relative position of these two genes is essential for normal regulation of fmdS.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.