ArticleThe Biochemical journal2001
Characterization of monocot and dicot plant S-adenosyl-l-methionine decarboxylase gene families including identification in the mRNA of a highly conserved pair of upstream overlapping open reading frames.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 99 citations in OpenAlex.
- TheGenome research · 2025Article
- Transcriptional repression ofPlant direct · 2024Article
- Heterografted chrysanthemums enhance salt stress tolerance by integrating reactive oxygen species, soluble sugar, and proline.Horticulture research · 2022Article
- TOR senses and regulates spermidine metabolism during seedling establishment and growth in maize and Arabidopsis.iScience · 2021Article
- Comprehensive genome-wide identification of angiosperm upstream ORFs with peptide sequences conserved in various taxonomic ranges using a novel pipeline, ESUCA.BMC genomics · 2020Article
- Complexity and Conservation of Thermospermine-Responsive uORFs ofFrontiers in plant science · 2019Article
- Polyamines in the life of Arabidopsis: profiling the expression of S-adenosylmethionine decarboxylase (SAMDC) gene family during its life cycle.BMC plant biology · 2017Article
- Molecular cloning and characterization ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2017Article
- uORF, a regulatory mechanism of the Arabidopsis polyamine oxidase 2.Molecular biology reports · 2014Article
- Arabidopsis ribosomal proteins control developmental programs through translational regulation of auxin response factors.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Known and novel post-transcriptional regulatory sequences are conserved across plant families.RNA (New York, N.Y.) · 2012Article
- Polyamine Homeostasis in Wild Type and Phenolamide Deficient Arabidopsis thaliana Stamens.Frontiers in plant science · 2012Article
- Molecular characterization of the Arginine decarboxylase gene family in rice.Transgenic research · 2010Article
- A profusion of upstream open reading frame mechanisms in polyamine-responsive translational regulation.Nucleic acids research · 2010Review
- Post-transcriptional regulation of gene expression in plants during abiotic stress.International journal of molecular sciences · 2009Review
- Review
- Conserved upstream open reading frames in higher plants.BMC genomics · 2008Article
- Advances in polyamine research in 2007.Journal of plant research · 2007Review
- The pivotal roles of the plant S-adenosylmethionine decarboxylase 5' untranslated leader sequence in regulation of gene expression at the transcriptional and posttranscriptional levels.Plant physiology · 2005Article
- A conserved upstream open reading frame mediates sucrose-induced repression of translation.The Plant cell · 2004Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
S-Adenosyl-L-methionine decarboxylase (AdoMetDC; EC 4.1.1.50) is one of the key regulatory enzymes in the biosynthesis of polyamines. Isolation of genomic and cDNA sequences from rice and Arabidopsis had indicated that this enzyme is encoded by a small multigene family in monocot and dicot plants. Analysis of rice, maize and Arabidopsis AdoMetDC cDNA species revealed that the monocot enzyme possesses an extended C-terminus relative to dicot and human enzymes. Interestingly, we discovered that all expressed plant AdoMetDC mRNA 5' leader sequences contain a highly conserved pair of overlapping upstream open reading frames (uORFs) that overlap by one base. The 5' tiny uORF consists of two or three codons and the 3' small uORF encodes 50-54 residues. Sequences of the small uORFs are highly conserved between monocot, dicot and gymnosperm AdoMetDC mRNA species and the C-terminus of the plant small uORFs is conserved with the C-terminus of nematode AdoMetDC uORFs; such a conserved arrangement is strongly suggestive of a translational regulatory mechanism. No introns were found in the main AdoMetDC proenzyme ORF from any of the plant genes encoding AdoMetDC, whereas introns were found in conserved positions flanking the overlapping uORFs. The absence of the furthest 3' intron from the Arabidopsis gene encoding AdoMetDC2 suggests that this intron was lost recently. Reverse-transcriptase-mediated PCR analysis of the two Arabidopsis genes for AdoMetDC indicated that AdoMetDC1 is abundant and ubiquitous, whereas the gene for AdoMetDC2 is expressed preferentially in leaves and inflorescences. Investigation of recently released Arabidopsis genome sequences has revealed that in addition to the two genes encoding AdoMetDC isolated as part of the present work, four additional genes are present in Arabidopsis but they are probably not expressed.
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