ArticleThe Biochemical journal2001
gamma-Interferon decreases the level of 26 S proteasomes and changes the pattern of phosphorylation.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 68 citations in OpenAlex.
- PI31 is a positive regulator of 20S immunoproteasome assembly.Journal of cell science · 2025Article
- Adaptation of the Tumor Antigen Presentation Machinery to Ionizing Radiation.Journal of immunology (Baltimore, Md. : 1950) · 2023Article
- Review
- Article
- PA28αβ overexpression enhances learning and memory of female mice without inducing 20S proteasome activity.BMC neuroscience · 2018Article
- Islet stress, degradation and autoimmunity.Diabetes, obesity & metabolism · 2018Review
- Reversible phosphorylation of the 26S proteasome.Protein & cell · 2017Review
- PA28αβ: the enigmatic magic ring of the proteasome?Biomolecules · 2014Review
- Differential roles of proteasome and immunoproteasome regulators Pa28αβ, Pa28γ and Pa200 in the degradation of oxidized proteins.Archives of biochemistry and biophysics · 2012Article
- Lipopolysaccharide-induced neuroinflammation leads to the accumulation of ubiquitinated proteins and increases susceptibility to neurodegeneration induced by proteasome inhibition in rat hippocampus.Journal of neuroinflammation · 2012Article
- Immunoproteasomes: structure, function, and antigen presentation.Progress in molecular biology and translational science · 2012Review
- Phosphorylation and methylation of proteasomal proteins of the haloarcheon Haloferax volcanii.Archaea (Vancouver, B.C.) · 2010Article
- Persistent hijacking of brain proteasomes in HIV-associated dementia.The American journal of pathology · 2010Article
- Two-dimensional electrophoresis-based characterization of post-translational modifications of mammalian 20S proteasome complexes.Proteomics · 2008Article
- Epidermal growth factor receptor vIII expression in U87 glioblastoma cells alters their proteasome composition, function, and response to irradiation.Molecular cancer research : MCR · 2008Article
- Mild heat stress stimulates 20S proteasome and its 11S activator in human fibroblasts undergoing aging in vitro.Cell stress & chaperones · 2004Article
- Phosphorylation of 20S proteasome alpha subunit C8 (alpha7) stabilizes the 26S proteasome and plays a role in the regulation of proteasome complexes by gamma-interferon.The Biochemical journal · 2004Article
- MEKK3 interacts with the PA28 gamma regulatory subunit of the proteasome.The Biochemical journal · 2003Article
- Functional regulation of immunoproteasomes and transporter associated with antigen processing.Immunologic research · 2001Review
- Aging and dietary restriction alter proteasome biogenesis and composition in the brain and liver.Mechanisms of ageing and developmentArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In mammalian cells proteasomes can be activated by two different types of regulatory complexes which bind to the ends of the proteasome cylinder. Addition of two 19 S (PA700; ATPase) complexes forms the 26 S proteasome, which is responsible for ATP-dependent non-lysosomal degradation of intracellular proteins, whereas 11 S complexes (PA28; REG) have been implicated in antigen processing. The PA28 complex is upregulated in response to gamma-interferon (gamma-IFN) as are three non-essential subunits of the 20 S proteasome. In the present study we have investigated the effects of gamma-IFN on the level of different proteasome complexes and on the phosphorylation of proteasome subunits. After treatment of cells with gamma-IFN, the level of 26 S proteasomes decreased and there was a concomitant increase in PA28-proteasome complexes. However, no free 19 S regulatory complexes were detected. The majority of the gamma-IFN-inducible proteasome subunits LMP2 and LMP7 were present in PA28-proteasome complexes, but these subunits were also found in 26 S proteasomes. The level of phosphorylation of both 20 S and 26 S proteasome subunits was found to decrease after gamma-IFN treatment of cells. The C8 alpha subunit showed more than a 50% decrease in phosphorylation, and the phosphorylation of C9 was only barely detectable after gamma-IFN treatment. These results suggest that association of regulatory components to 20 S proteasomes is regulated, and that phosphorylation of proteasome alpha subunits may be one mode of regulation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.