ArticleBritish journal of cancer2001
Evidence against apoptosis as a major mechanism for reproductive cell death following treatment of cell lines with anti-cancer drugs.
Article in British journal of cancer, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 63 citations in OpenAlex.
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- An Insight into Synthetic Strategies, SAR Study and Anticancer Mechanism of Chalcone Derivatives: Medicinal Chemistry Perspective.Current drug research reviews · 2025Review
- DNA Damage-Response Pathway Heterogeneity of Human Lung Cancer A549 and H1299 Cells Determines Sensitivity to 8-Chloro-Adenosine.International journal of molecular sciences · 2018Article
- Cytotoxic and toxicogenomic effects of silibinin in bladder cancer cells with different TP53 status.Journal of biosciences · 2017Article
- Mechanisms of Drug Resistance in Veterinary Oncology- A Review with an Emphasis on Canine Lymphoma.Veterinary sciences · 2015Review
- Gemcitabine impacts differentially on bladder and kidney cancer cells: distinct modulations in the expression patterns of apoptosis-related microRNAs and BCL2 family genes.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2015Article
- Linking the history of radiation biology to the hallmarks of cancer.Radiation research · 2014Review
- Apoptosis in Living Animals Is Assisted by Scavenger Cells and Thus May Not Mainly Go through the Cytochrome C-Caspase Pathway.Journal of Cancer · 2013Article
- Determine the effect of p53 on chemosensitivity.Methods in molecular biology (Clifton, N.J.) · 2013Article
- Quantitative immunohistochemistry for evaluating the distribution of Ki67 and other biomarkers in tumor sections and use of the method to study repopulation in xenografts after treatment with paclitaxel.Neoplasia (New York, N.Y.) · 2012Article
- Cytotoxic effects of the novel isoflavone, phenoxodiol, on prostate cancer cell lines.Journal of biosciences · 2012Article
- Apoptosis and survival.Indian journal of human genetics · 2011Article
- The role of apoptotic cell death in the radiosensitising effect of gemcitabine.British journal of cancer · 2009Article
- Could 99mTc-glucarate be used to evaluate tumour necrosis? In vitro and in vivo studies in leukaemic tumour cell line U937.European journal of nuclear medicine and molecular imaging · 2008Article
- Article
- Cell death in irradiated prostate epithelial cells: role of apoptotic and clonogenic cell kill.Prostate cancer and prostatic diseases · 2003Article
- Possible direct cytoxicity effects of cyclophosphamide on cultured human follicles: an electron microscopy study.Journal of assisted reproduction and genetics · 2002Article
- Evaluation of 2-deoxy-D-glucose as a chemotherapeutic agent: mechanism of cell death.British journal of cancer · 2002Article
- Recurrent growth factor starvation promotes drug resistance in human leukaemic cells.British journal of cancer · 2002Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An increase in apoptotic cells may be observed after treatment with chemotherapy, and many authors have assumed that anti-cancer drugs kill cells by inducing apoptosis. The most relevant endpoint of cell death following treatment of tumour cells is loss of reproductive ability as measured by a colony-forming assay, since cells with limited reproductive potential cannot regenerate a tumour. We have therefore investigated the relationship between apoptosis and reproductive cell death following in vitro treatment of mammalian cell lines with anti-cancer drugs. Markers of apoptosis (DNA ladders, TUNEL assay) were evaluated at various times after treatment of Chinese Hamster Ovary (CHO) cells, human bladder cancer MGH-U1 cells, and a murine T-lymphocytic cell line (CTLL-2) with several anti-cancer drugs. These markers were found infrequently, despite the use of doses that cause loss of colony-forming ability, except in CTLL-2 cells. We also transfected and expressed the human pro-apoptotic gene bax and the anti-apoptotic gene bcl-2 in MGH-U1 cells and compared cell survival after drug treatment with that of control cells transfected with the vector alone. Expression of these genes had at most small effects to influence cell survival. We conclude that apoptotic mechanisms had at most a minor role in leading to reproductive death of MGH-U1 and CHO cells after chemotherapy. When apoptosis is observed following treatment with anti-cancer drugs it may be a secondary event which occurs in lethally-damaged cells, leading to their lysis, rather than a primary event that leads to loss of reproductive integrity.
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