ArticleClinical and diagnostic laboratory immunology2001
Identifying diagnostic peptides for lyme disease through epitope discovery.
Article in Clinical and diagnostic laboratory immunology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 71 citations in OpenAlex.
- Phage Display Against 2D Metal-Organic Nanosheets as a New Route to Highly Selective Biomolecular Recognition Surfaces.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- New Epitopes for the Serodiagnosis of Human Borreliosis.Microorganisms · 2024Article
- Identification and Structure of Epitopes on Cashew Allergens Ana o 2 and Ana o 3 Using Phage Display.Molecules (Basel, Switzerland) · 2023Article
- Recent Advances in the Immunologic Method Applied to Tick-Borne Diseases in Brazil.Pathogens (Basel, Switzerland) · 2022Review
- Identification of a novel B cell epitope on the nucleocapsid protein of porcine deltacoronavirus.Virus research · 2021Article
- Identification of Two Novel Linear Neutralizing Epitopes within the Hexon Protein of Canine Adenovirus Using Monoclonal Antibodies.Vaccines · 2021Article
- Characterization of Monoclonal Antibodies against σA Protein and Cross-Reactive Epitope Identification and Application for Detection of Duck and Chicken Reovirus Infections.Pathogens (Basel, Switzerland) · 2019Article
- Review
- Zika Virus Specific Diagnostic Epitope Discovery.Journal of visualized experiments : JoVE · 2017Article
- Immunocontraception: Filamentous Bacteriophage as a Platform for Vaccine Development.Current medicinal chemistry · 2017Review
- Antibody Response to Lyme Disease Spirochetes in the Context of VlsE-Mediated Immune Evasion.Infection and immunity · 2017Article
- Improving serodiagnosis of human and canine leishmaniasis with recombinant Leishmania braziliensis cathepsin l-like protein and a synthetic peptide containing its linear B-cell epitope.PLoS neglected tropical diseases · 2015Article
- Identification of a conserved B-cell epitope on duck hepatitis A type 1 virus VP1 protein.PloS one · 2015Article
- Identification of a novel Haemophilus parasuis-specific B cell epitope using monoclonal antibody against the OppA protein.PloS one · 2014Article
- Antibody biomarker discovery through in vitro directed evolution of consensus recognition epitopes.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Antibody repertoire profiling using bacterial display identifies reactivity signatures of celiac disease.Analytical chemistry · 2013Article
- Article
- Identification of peptide mimotopes of Trypanosoma brucei gambiense variant surface glycoproteins.PLoS neglected tropical diseases · 2011Article
- Mimotope ELISA for detection of broad spectrum antibody against avian H5N1 influenza virus.PloS one · 2011Article
- Applying bioinformatics for antibody epitope prediction using affinity-selected mimotopes - relevance for vaccine design.Immunome research · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Serum antibodies from patients with Lyme disease (LD) were used to affinity select peptide epitopes from 12 large random peptide libraries in phage display format. The selected peptides were surveyed for reactivity with a panel of positive sera (from LD patients) and negative sera (from subjects without LD), thus identifying 17 peptides with a diagnostically useful binding pattern: reactivity with at least three positive sera and no reactivity with any of the negative sera. The peptides define eight sequence motifs, none of which can be matched convincingly with segments of proteins from Borrelia burgdorferi, the LD pathogen; evidently, then, they are "mimotopes," mimicking natural pathogen epitopes without matching contiguous amino acids of pathogen proteins. Peptides like these could be the basis of a new diagnostic enzyme-linked immunosorbent assay for LD, with sufficient specificity and sensitivity to replace expensive immunoblotting tests that are currently required for definitive serological diagnosis. Moreover, the method used to discover these peptides did not require any knowledge of the pathogen and involved generic procedures that are applicable to almost any infectious disease, including emerging diseases for which no pathogen has yet been identified.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.