ArticleThe Journal of physiology2001
The amino side of the C-terminus determines fast inactivation of the T-type calcium channel alpha1G.
Article in The Journal of physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 64 citations in OpenAlex.
- Calcium and arrhythmogenesis.Physiological reviews · 2007Pooled it
- T-Type Voltage-Gated Calcium Channels: Potential Regulators of Smooth Muscle Contractility.International journal of molecular sciences · 2024Review
- Selective inhibition of neuronal CaMolecular brain · 2020Article
- Identification of a molecular gating determinant within the carboxy terminal region of CaMolecular brain · 2019Article
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- Amazing T-type calcium channels: updating functional properties in health and disease.Pflugers Archiv : European journal of physiology · 2014Review
- Cooperative activation of the T-type CaV3.2 channel: interaction between Domains II and III.The Journal of biological chemistry · 2013Article
- Three-dimensional structure of CaV3.1: comparison with the cardiac L-type voltage-gated calcium channel monomer architecture.The Journal of biological chemistry · 2009Article
- A Cav3.2 T-type calcium channel point mutation has splice-variant-specific effects on function and segregates with seizure expression in a polygenic rat model of absence epilepsy.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- I-II loop structural determinants in the gating and surface expression of low voltage-activated calcium channels.PloS one · 2008Article
- The effect of the outermost basic residues in the S4 segments of the Ca(V)3.1 T-type calcium channel on channel gating.Pflugers Archiv : European journal of physiology · 2007Article
- The I-II loop controls plasma membrane expression and gating of Ca(v)3.2 T-type Ca2+ channels: a paradigm for childhood absence epilepsy mutations.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Evidence for common structural determinants of activation and inactivation in T-type Ca2+ channels.Pflugers Archiv : European journal of physiology · 2006Article
- Role of Domain IV/S4 outermost arginines in gating of T-type calcium channels.Pflugers Archiv : European journal of physiology · 2005Article
- Y3+ block demonstrates an intracellular activation gate for the alpha1G T-type Ca2+ channel.The Journal of general physiology · 2004Article
- Mechanism of arachidonic acid modulation of the T-type Ca2+ channel alpha1G.The Journal of general physiology · 2004Article
- Slow inactivation of the Ca(V)3.1 isotype of T-type calcium channels.The Journal of physiology · 2004Article
- Functional roles of cytoplasmic loops and pore lining transmembrane helices in the voltage-dependent inactivation of HVA calcium channels.The Journal of physiology · 2004Review
- Calcium channels: unanswered questions.Journal of bioenergetics and biomembranes · 2003Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We analysed the kinetic properties of the fast inactivating T-type calcium channel alpha1G in HEK 293 cells transfected with different alpha1G chimeras, containing the N-terminus, III-IV linker or various C-terminal regions of the slowly inactivating L-type alpha1C. A highly negatively charged region of 23 amino acids at the amino side of the intracellular carboxy terminus of alpha1G was found to be critical for fast inactivation. The N-terminus of alpha1G does not seem to be necessary for inactivation of the T-type calcium channel because replacement of the a1G N-terminus with the alpha1C N-terminus did not influence channel kinetics at all. Replacing the III-IV linker of alpha1G with that of a1C decreased the rate of inactivation at -20 mV from 15.8 +/- 1.8 to 8.5 +/- 1.1 ms, and shifted the potential for half-maximal inactivation from -69.6 +/- 0.8 to -54.0 +/- 1.7 mV. However, these parameters were not significantly different at other potentials. We suggest a putative 'ball-and-chain'-like mechanism for inactivation in which the negative charges function as an acceptor domain for a ball, hypothetically located at a different intracellular part of the channel. In addition, transferring the IQ motif and EF hand of alpha1C to alpha1G does not confer Ca2+-dependent inactivation on alpha1G, suggesting that other sequences besides the C-terminus are needed for Ca2+-dependent inactivation of alpha1C.
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