ArticleThe Journal of physiology2001
Regulation of Drosophila transient receptor potential-like (TrpL) channels by phospholipase C-dependent mechanisms.
Article in The Journal of physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 134 citations in OpenAlex.
- Structural basis of TRPV1 modulation by endogenous bioactive lipids.Nature structural & molecular biology · 2024Article
- Diacylglycerol Activates theInternational journal of molecular sciences · 2023Article
- The Role of Membrane Lipids in Light-Activation ofBiomolecules · 2022Review
- Methods to study phosphoinositide regulation of ion channels.Methods in enzymology · 2021Article
- Transduction and Adaptation Mechanisms in the Cilium or Microvilli of Photoreceptors and Olfactory Receptors From Insects to Humans.Frontiers in cellular neuroscience · 2021Review
- Phosphatidylinositol 4,5-bisphosphate directly interacts with the β and γ subunits of the sodium channel ENaC.The Journal of biological chemistry · 2020Article
- Molecular basis for the sensitivity of TRP channels to polyunsaturated fatty acids.Naunyn-Schmiedeberg's archives of pharmacology · 2018Article
- Phosphoinositide regulation of TRPV1 revisited.Pflugers Archiv : European journal of physiology · 2015Review
- Lack of Dietary Polyunsaturated Fatty Acids Causes Synapse Dysfunction in the Drosophila Visual System.PloS one · 2015Article
- Diacylglycerol activates the light-dependent channel TRP in the photosensitive microvilli of Drosophila melanogaster photoreceptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Phosphoinositide regulation of TRP channels.Handbook of experimental pharmacology · 2014Review
- Regulation of transient receptor potential channels by the phospholipase C pathway.Advances in biological regulation · 2013Review
- Phosphoinositides: tiny lipids with giant impact on cell regulation.Physiological reviews · 2013Review
- Ambient temperature affects the temperature threshold for TRPM8 activation through interaction of phosphatidylinositol 4,5-bisphosphate.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- TRPV1 channels are intrinsically heat sensitive and negatively regulated by phosphoinositide lipids.Neuron · 2013Article
- TRPC5 channel is the mediator of neurotrophin-3 in regulating dendritic growth via CaMKIIα in rat hippocampal neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Drosophila visual transduction.Trends in neurosciences · 2012Review
- Signal-dependent hydrolysis of phosphatidylinositol 4,5-bisphosphate without activation of phospholipase C: implications on gating of Drosophila TRPL (transient receptor potential-like) channel.The Journal of biological chemistry · 2012Article
- Article
- Melanopsin-expressing amphioxus photoreceptors transduce light via a phospholipase C signaling cascade.PloS one · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Patch clamp and fura-2 fluorescence were employed to characterize receptor-mediated activation of recombinant Drosophila TrpL channels expressed in Sf9 insect cells. TrpL was activated by receptor stimulation and by exogenous application of diacylglycerol (DAG) or poly-unsaturated fatty acids (PUFAs). Activation of TrpL was blocked more than 70% by U73122, suggesting that the effect of these agents was dependent upon phospholipase C (PLC). In fura-2 assays, extracellular application of bacterial phosphatidylinositol (PI)-PLC or phosphatidylcholine (PC)-PLC caused a transient increase in TrpL channel activity, the magnitude of which was significantly less than that observed following receptor stimulation. TrpL channels were also activated in excised inside-out patches by cytoplasmic application of mammalian PLC-b2, bacterial PI-PLC and PC-PLC, but not by phospholipase D (PLD). The phospholipases had little or no effect when examined in either whole-cell or cell-attached configurations.TrpL activity was inhibited by addition of phosphatidylinositol-4,5-bisphosphate (PIP2) to excised inside-out membrane patches exhibiting spontaneous channel activity or to patches pre-activated by treatment with PLC. The effect was reversible, specific for PIP2, and was not observed with phosphatidylethanolamine (PE), PI, PC or phosphatidylserine (PS). However, antibodies against PIP2 consistently failed to activate TrpL in inside-out patches. It is concluded that both the hydrolysis of PIP2 and the generation of DAG are required to rapidly activate TrpL following receptor stimulation, or that some other PLC-dependent mechanism plays a crucial role in the activation process.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.