ArticleJournal of clinical microbiology2001
Simultaneous detection and typing of influenza viruses A and B by a nested reverse transcription-PCR: comparison to virus isolation and antigen detection by immunofluorescence and optical immunoassay (FLU OIA).
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 128 citations in OpenAlex.
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- Nanoisland SERS-Substrates for Specific Detection and Quantification of Influenza A Virus.Biosensors · 2023Article
- Multiplex Lithographic SERS Aptasensor for Detection of Several Respiratory Viruses in One Pot.International journal of molecular sciences · 2023Article
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- Aptamer-coated track-etched membranes with a nanostructured silver layer for single virus detection in biological fluids.Frontiers in bioengineering and biotechnology · 2022Article
- A Combination of Membrane Filtration and Raman-Active DNA Ligand Greatly Enhances Sensitivity of SERS-Based Aptasensors for Influenza A Virus.Frontiers in chemistry · 2022Article
- Integrated Microfluidic Preconcentration and Nucleic Amplification System for Detection of Influenza A Virus H1N1 in Saliva.Micromachines · 2020Article
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- Molecular characterization of influenza viruses collected from young children in Uberlandia, Brazil - from 2001 to 2010.BMC infectious diseases · 2015Article
- Review
- Human metapneumovirus-associated hospital admissions over five consecutive epidemic seasons: evidence for alternating circulation of different genotypes.Journal of medical virology · 2012Article
- High-throughput detection method for influenza virus.Journal of visualized experiments : JoVE · 2012Article
- Multiplexed, rapid detection of H5N1 using a PCR-free nanoparticle-based genomic microarray assay.BMC biotechnology · 2010Article
- Nucleic acid dipstick test for molecular diagnosis of pandemic H1N1.Journal of clinical microbiology · 2010Article
- Broadly targeted triplex real-time PCR detection of influenza A, B and C viruses based on the nucleoprotein gene and a novel "MegaBeacon" probe strategy.Journal of virological methods · 2010Article
- Article
- Broadly targeted multiprobe QPCR for detection of coronaviruses: Coronavirus is common among mallard ducks (Anas platyrhynchos).Journal of virological methods · 2009Article
- Testing of diagnostic methods for detection of influenza virus for optimal performance in the context of an influenza surveillance network.Journal of clinical microbiology · 2007Article
- Comparison of the MChip to viral culture, reverse transcription-PCR, and the QuickVue influenza A+B test for rapid diagnosis of influenza.Journal of clinical microbiology · 2007Article
- Laboratory diagnosis of human seasonal and pandemic influenza virus infection.The Medical journal of Australia · 2006Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A nested reverse transcription (RT)-PCR was developed for simultaneous detection and typing of influenza viruses A and B. The detection limit for influenza virus A subtypes H1 and H3 and that for influenza virus B were between 1 and 4 target gene copies per reaction for each type. The clinical benefit of the RT-PCR method was evaluated by comparing the results with virus isolation and direct immunofluorescence (IF) assays on 215 nasopharyngeal aspirates from patients with suspected influenza virus infection. The RT-PCR detected 83 cases of influenza A, compared to 66 cases detected by virus isolation and 68 cases detected by IF assay. The corresponding figures for the detection of influenza B were 15, 12, and 11 cases, respectively. In total, 16 out of 98 RT-PCR-positive specimens were negative by virus isolation and IF. An optical immunoassay for rapid detection of influenza A and B (FLU OIA; Bio Star Inc., Boulder, Colo.) was compared to RT-PCR and IF on 105 nasopharyngeal aspirates and 79 swabs. The sensitivity for the OIA was 40.4% compared to PCR and 48.8% compared to IF assay, when nasopharyngeal aspirates were examined. The specificities were 94.3 and 93.9%, respectively. The sensitivity was higher for OIA on nasopharyngeal swabs, 77.5% and 86.6% compared to PCR and IF, respectively, while the specificity was lower, 82.0% and 75.5%, respectively. The RT-PCR provides a sensitive and specific method for detecting and typing influenza viruses A and B. The rapid OIA is useful as a complementary test, but it cannot replace established methods without further evaluation.
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