ArticleJournal of clinical microbiology2001
Evaluation of pulsed-field gel electrophoresis in epidemiological investigations of meningococcal disease outbreaks caused by Neisseria meningitidis serogroup C.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Using Neisseria meningitidis genomic diversity to inform outbreak strain identification.PLoS pathogens · 2021Article
- Whole genome sequencing for investigations of meningococcal outbreaks in the United States: a retrospective analysis.Scientific reports · 2018Article
- Distinct evolutionary patterns of Neisseria meningitidis serogroup B disease outbreaks at two universities in the USA.Microbial genomics · 2018Article
- Pulsotype Diversity of Clostridium botulinum Strains Containing Serotypes A and/or B Genes.Foodborne pathogens and disease · 2017Article
- The Application of Pulsed Field Gel Electrophoresis in Clinical Studies.Journal of clinical and diagnostic research : JCDR · 2016Review
- Article
- First Use of a Serogroup B Meningococcal Vaccine in the US in Response to a University Outbreak.Pediatrics · 2015Article
- Clonal analysis of Neisseria meningitidis serogroup B strains in South Africa, 2002 to 2006: emergence of new clone ST-4240/6688.Journal of clinical microbiology · 2012Article
- Phenotypic and molecular characterization of serogroup C Neisseria meningitidis associated with an outbreak in Bahia, Brazil.Enfermedades infecciosas y microbiologia clinica · 2012Article
- Phenotypic and molecular characterization of invasive serogroup W135 Neisseria meningitidis strains from 1990 to 2005 in Brazil.The Journal of infection · 2010Article
- Cluster of serogroup W135 meningococci, southeastern Florida, 2008-2009.Emerging infectious diseases · 2010Article
- Molecular epidemiology of Neisseria meningitidis isolates from an outbreak of meningococcal disease among men who have sex with men, Chicago, Illinois, 2003.Journal of clinical microbiology · 2007Article
- Usefulness of pulsed-field gel electrophoresis in tracking two outbreaks of invasive meningococcal disease serogroup C in British Columbia.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2007Article
- Ciprofloxacin-resistant Neisseria meningitidis, Delhi, India.Emerging infectious diseases · 2007Article
- Analysis of phenotypic variants of the serogroup C ET-15 clone of Neisseria meningitidis by pulsed-field gel electrophoresis.Journal of clinical microbiology · 2007Article
- Meningococcal disease in South Africa, 1999-2002.Emerging infectious diseases · 2007Article
- Use of a multilocus variable-number tandem repeat analysis method for molecular subtyping and phylogenetic analysis of Neisseria meningitidis isolates.BMC microbiology · 2006Article
- Molecular epidemiology and emergence of worldwide epidemic clones of Neisseria meningitidis in Taiwan.BMC infectious diseases · 2006Article
- Prospects for vaccine prevention of meningococcal infection.Clinical microbiology reviews · 2006Review
- Clonal spread of serogroup W135 meningococcal disease in Turkey.Journal of clinical microbiology · 2006Article
Corrections and comments
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Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since 1990, the frequency of Neisseria meningitidis serogroup C (NMSC) outbreaks in the United States has increased. Based on multilocus enzyme electrophoresis (MEE), the current molecular subtyping standard, most of the NMSC outbreaks have been caused by isolates of several closely related electrophoretic types (ETs) within the ET-37 complex. We chose 66 isolates from four well-described NMSC outbreaks that occurred in the United States from 1993 to 1995 to evaluate the potential of pulsed-field gel electrophoresis (PFGE) to identify outbreak-related isolates specific for each of the four outbreaks and to differentiate between them and 50 sporadic isolates collected during the outbreak investigations or through active laboratory-based surveillance from 1989 to 1996. We tested all isolates collected during the outbreak investigations by four other molecular subtyping methods: MEE, ribotyping (ClaI), random amplified polymorphic DNA assay (two primers), and serotyping and serosubtyping. Among the 116 isolates, we observed 11 clusters of 39 NheI PFGE patterns. Excellent correlation between the PFGE and the epidemiological data was observed, with an overall sensitivity of 85% and specificity of 71% at the 95% pattern relatedness breakpoint using either 1.5 or 1.0% tolerance. For all four analyzed outbreaks, PFGE would have given public health officials additional support in declaring an outbreak and making appropriate public health decisions.
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