ArticleMolecular microbiology2001
Cytochrome oxidase subunit VI of Trypanosoma brucei is imported without a cleaved presequence and is developmentally regulated at both RNA and protein levels.
Article in Molecular microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 23 citations in OpenAlex.
- A post-transcriptional respiratome regulon in trypanosomes.Nucleic acids research · 2019Article
- Mitochondrial and nucleolar localization of cysteine desulfurase Nfs and the scaffold protein Isu in Trypanosoma brucei.Eukaryotic cell · 2014Article
- Novel role of calmodulin in regulating protein transport to mitochondria in a unicellular eukaryote.Molecular and cellular biology · 2013Article
- Euglena gracilis and Trypanosomatids possess common patterns in predicted mitochondrial targeting presequences.Journal of molecular evolution · 2012Article
- Mitochondrial membrane complex that contains proteins necessary for tRNA import in Trypanosoma brucei.The Journal of biological chemistry · 2012Article
- The Trypanosoma brucei MitoCarta and its regulation and splicing pattern during development.Nucleic acids research · 2010Article
- A split and rearranged nuclear gene encoding the iron-sulfur subunit of mitochondrial succinate dehydrogenase in Euglenozoa.BMC research notes · 2009Article
- Post-transcriptional control of nuclear-encoded cytochrome oxidase subunits in Trypanosoma brucei: evidence for genome-wide conservation of life-cycle stage-specific regulatory elements.Nucleic acids research · 2006Article
- Import and assembly of Neurospora crassa Tom40 into mitochondria of Trypanosoma brucei in vivo.Current genetics · 2003Article
- Mitochondrial development during life cycle differentiation of African trypanosomes: evidence for a kinetoplast-dependent differentiation control point.Molecular biology of the cell · 2002Article
- A novel CCCH protein which modulates differentiation of Trypanosoma brucei to its procyclic form.The EMBO journal · 2001Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Mitochondrial respiration in the African trypanosome undergoes dramatic developmental stage regulation. This requires co-ordinated control of components encoded by both the nuclear genome and the kinetoplast, the unusual mitochondrial genome of these parasites. As a model for understanding the co-ordination of these genomes, we have examined the regulation and mitochondrial import of a nuclear-encoded component of the cytochrome oxidase complex, cytochrome oxidase subunit VI (COXVI). By generating transgenic trypanosomes expressing intact or mutant forms of this protein, we demonstrate that COXVI is not imported using a conventional cleaved presequence and show that sequences at the N-terminus of the protein are necessary for correct mitochondrial sorting. Analyses of endogenous and transgenic COXVI mRNA and protein expression in parasites undergoing developmental stage differentiation demonstrates a temporal order of control involving regulation in the abundance of, first, mRNA and then protein. This represents the first dissection of the regulation and import of a nuclear-encoded protein into the cytochrome oxidase complex in these organisms, which were among the earliest eukaryotes to possess a mitochondrion.
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