ArticleProceedings of the National Academy of Sciences of the United States of America2001
Multiplex allele-specific target amplification based on PCR suppression.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- AAV vector-derived elements integrate into Cas9-generated double-strand breaks and disrupt gene transcription.Molecular therapy : the journal of the American Society of Gene Therapy · 2024Article
- Suppression PCR-Based Selective Enrichment Sequencing for Pathogen and Antimicrobial Resistance Detection on Cell-Free DNA in Sepsis-A Targeted, Blood Culture-Independent Approach for Rapid Pathogen and Resistance Diagnostics in Septic Patients.International journal of molecular sciences · 2024Article
- Novel CRISPR-based sequence specific enrichment methods for target loci and single base mutations.PloS one · 2020Article
- A Diagnostic Device for In-Situ Detection of Swine Viral Diseases: The SWINOSTICS Project.Sensors (Basel, Switzerland) · 2019Article
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- Analysis of genetic variations in CYP2C9, CYP2C19, CYP2D6 and CYP3A5 genes using oligonucleotide microarray.International journal of clinical and experimental medicine · 2015Article
- Transduction-specific ATLAS reveals a cohort of highly active L1 retrotransposons in human populations.Human mutation · 2013Article
- Highly rapid amplification-free and quantitative DNA imaging assay.Scientific reports · 2013Article
- Diversity arrays technology (DArT) markers in apple for genetic linkage maps.Molecular breeding : new strategies in plant improvement · 2012Article
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- Rapid PCR-based molecular pathotyping of H5 and H7 avian influenza viruses.Journal of clinical microbiology · 2011Article
- The evolutionary-developmental analysis of plant microRNAs.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2010Article
- Chemically modified primers for improved multiplex polymerase chain reaction.Analytical biochemistry · 2009Article
- Multigene amplification and massively parallel sequencing for cancer mutation discovery.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Multiplex amplification of all coding sequences within 10 cancer genes by Gene-Collector.Nucleic acids research · 2007Article
- Validation of the high-throughput marker technology DArT using the model plant Arabidopsis thaliana.Molecular genetics and genomics : MGG · 2005Article
- Detection of virulence genes in Malaysian Shigella species by multiplex PCR assay.BMC infectious diseases · 2005Article
- MARA: a novel approach for highly multiplexed locus-specific SNP genotyping using high-density DNA oligonucleotide arrays.Nucleic acids research · 2004Article
- Microarray-based detection and genotyping of viral pathogens.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- SNP genotyping by multiplexed solid-phase amplification and fluorescent minisequencing.Genome research · 2001Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a strategy for multiplex PCR based on PCR suppression. PCR suppression allows DNA target amplification with only one sequence-specific primer per target and a second primer that is common for all targets. Therefore, an n-plex PCR would require only n + 1 primers. We have demonstrated uniform, efficient amplification of targeted sequences in 14-plex PCR. The high specificity of suppression PCR also provides multiplexed amplification with allele specificity. Multiplexed PCR was used to develop assays for genotyping DNA samples from cystic fibrosis-affected individuals. The new approach greatly simplifies primer design, significantly increases the PCR multiplexing level, and decreases the overall primer cost. In addition, this assay is more readily amenable to automation and is therefore suitable for high-throughput genetic diagnostics.
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