ArticleProceedings of the National Academy of Sciences of the United States of America2001
Metabolic engineering of plant alkaloid biosynthesis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 210 citations in OpenAlex.
- A Comprehensive Review on the Biosynthesis of Tropane Alkaloids.Molecules (Basel, Switzerland) · 2026Review
- Isolation and characterization of Saccharomyces cerevisiae mutants with ornithine accumulation for value-added craft beer brewing.Journal of industrial microbiology & biotechnology · 2026Article
- Neuroprotective mechanisms of nutraceutical berberine: Preclinical evidence and future perspectives.Advances in neurotoxicology · 2026Article
- Genetic regulation and manipulation of nicotine biosynthesis in tobacco: strategies to eliminate addictive alkaloids.Journal of experimental botany · 2024Review
- California poppy (Frontiers in plant science · 2023Review
- Proteomics and Co-expression Network Analysis Reveal the Importance of Hub Proteins and Metabolic Pathways in Nicotine Synthesis and Accumulation in Tobacco (Frontiers in plant science · 2022Article
- CRISPR/Cas9 technology for improving agronomic traits and future prospective in agriculture.Planta · 2021Review
- Impact of nicotine pathway downregulation on polyamine biosynthesis and leaf ripening in tobacco.Plant direct · 2021Article
- Involvement of the miR156/SPL module in flooding response in Medicago sativa.Scientific reports · 2021Article
- Article
- Engineering a microbial biosynthesis platform for de novo production of tropane alkaloids.Nature communications · 2019Article
- Fruit Salad in the Lab: Comparing Botanical Species to Help Deciphering Fruit Primary Metabolism.Frontiers in plant science · 2019Review
- Silencing of quinolinic acid phosphoribosyl transferase (QPT) gene for enhanced production of scopolamine in hairy root culture of Duboisia leichhardtii.Scientific reports · 2018Article
- Metabolic Engineering of Glycyrrhizin Pathway by Over-Expression of Beta-amyrin 11-Oxidase in Transgenic Roots of Glycyrrhiza glabra.Molecular biotechnology · 2018Article
- Green Routes for the Production of Enantiopure Benzylisoquinoline Alkaloids.International journal of molecular sciences · 2017Review
- Genetically engineered hairy root cultures of Hyoscyamus senecionis and H. muticus: ploidy as a promising parameter in the metabolic engineering of tropane alkaloids.Plant cell reports · 2017Article
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- Tyrosine phosphorylation and protein degradation control the transcriptional activity of WRKY involved in benzylisoquinoline alkaloid biosynthesis.Scientific reports · 2016Article
- Characterization of the Promoter Region of Biosynthetic Enzyme Genes Involved in Berberine Biosynthesis in Coptis japonica.Frontiers in plant science · 2016Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant alkaloids, one of the largest groups of natural products, provide many pharmacologically active compounds. Several genes in the biosynthetic pathways for scopolamine, nicotine, and berberine have been cloned, making the metabolic engineering of these alkaloids possible. Expression of two branching-point enzymes was engineered: putrescine N-methyltransferase (PMT) in transgenic plants of Atropa belladonna and Nicotiana sylvestris and (S)-scoulerine 9-O-methyltransferase (SMT) in cultured cells of Coptis japonica and Eschscholzia californica. Overexpression of PMT increased the nicotine content in N. sylvestris, whereas suppression of endogenous PMT activity severely decreased the nicotine content and induced abnormal morphologies. Ectopic expression of SMT caused the accumulation of benzylisoquinoline alkaloids in E. californica. The prospects and limitations of engineering plant alkaloid metabolism are discussed.
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