ArticleMolecular and cellular biology2001
Loss of orphan receptor germ cell nuclear factor function results in ectopic development of the tail bud and a novel posterior truncation.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 108 citations in OpenAlex.
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- The nuclear receptor NR6A1 plays an oncogenic role through reprogramming glycolysis in tumors.Biological research · 2025Article
- Variants in NR6A1 cause a novel oculo vertebral renal syndrome.Nature communications · 2025Article
- The expanding roles of Nr6a1 in development and evolution.Frontiers in cell and developmental biology · 2024Review
- International Union of Basic and Clinical Pharmacology CXIII: Nuclear Receptor Superfamily-Update 2023.Pharmacological reviews · 2023Review
- Brain nuclear receptors and cardiovascular function.Cell & bioscience · 2023Review
- Nr6a1 controls Hox expression dynamics and is a master regulator of vertebrate trunk development.Nature communications · 2022Article
- Regulation of Oct4 in stem cells and neural crest cells.Birth defects research · 2022Review
- Evidence for and localization of proposed causative variants in cattle and pig genomes.Genetics, selection, evolution : GSE · 2021Review
- Characterizing modifier genes of cardiac fibrosis phenotype in hypertrophic cardiomyopathy.International journal of cardiology · 2021Article
- Identification of novel susceptibility loci for non-syndromic cleft lip with or without cleft palate.Journal of cellular and molecular medicine · 2020Article
- Association analysis of polymorphism in the NR6A1 gene with the lumbar vertebrae number traits in sheep.Genes & genomics · 2019Article
- Nuclear receptors in neural stem/progenitor cell homeostasis.Cellular and molecular life sciences : CMLS · 2017Review
- A Structural Investigation into Oct4 Regulation by Orphan Nuclear Receptors, Germ Cell Nuclear Factor (GCNF), and Liver Receptor Homolog-1 (LRH-1).Journal of molecular biology · 2016Article
- Germ Cell Nuclear Factor (GCNF) Represses Oct4 Expression and Globally Modulates Gene Expression in Human Embryonic Stem (hES) Cells.The Journal of biological chemistry · 2016Article
- Germ Cell Nuclear Factor (GCNF/RTR) Regulates Transcription of Gonadotropin-Regulated Testicular RNA Helicase (GRTH/DDX25) in Testicular Germ Cells--The Androgen Connection.Molecular endocrinology (Baltimore, Md.) · 2015Article
- Independent regulation of vertebral number and vertebral identity by microRNA-196 paralogs.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- GCNF-dependent activation of cyclin D1 expression via repression of Mir302a during ESC differentiation.Stem cells (Dayton, Ohio) · 2014Article
- Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The dynamic embryonic expression of germ cell nuclear factor (GCNF), an orphan nuclear receptor, suggests that it may play an important role during early development. To determine the physiological role of GCNF, we have generated a targeted mutation of the GCNF gene in mice. Germ line mutation of the GCNF gene proves that the orphan nuclear receptor is essential for embryonic survival and normal development. GCNF(-/-) embryos cannot survive beyond 10.5 days postcoitum (dpc), probably due to cardiovascular failure. Prior to death, GCNF(-/-) embryos suffer significant defects in posterior development. Unlike GCNF(+/+) embryos, GCNF(-/-) embryos do not turn and remain in a lordotic position, the majority of the neural tube remains open, and the hindgut fails to close. GCNF(-/-) embryos also suffer serious defects in trunk development, specifically in somitogenesis, which terminates by 8.75 dpc. The maximum number of somites in GCNF(-/-) embryos is 13 instead of 25 as in the GCNF(+/+) embryos. Interestingly, the tailbud of GCNF(-/-) embryos develops ectopically outside the yolk sac. Indeed, alterations in expression of multiple marker genes were identified in the posterior of GCNF(-/-) embryos, including the primitive streak, the node, and the presomitic mesoderm. These results suggest that GCNF is required for maintenance of somitogenesis and posterior development and is essential for embryonic survival. These results suggest that GCNF regulates a novel and critical developmental pathway involved in normal anteroposterior development.
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