ArticleMolecular and cellular biology2001
Lipopolysaccharide-induced activation of beta2-integrin function in macrophages requires Irak kinase activity, p38 mitogen- activated protein kinase, and the Rap1 GTPase.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 92 citations in OpenAlex.
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- MARCKS Inhibition Alters Bovine Neutrophil Responses toBiomedicines · 2024Article
- SILAC-based quantitative proteomics to investigate the eicosanoid associated inflammatory response in activated macrophages.Journal of inflammation (London, England) · 2022Article
- Mechanistic consideration of the effect of perioperative volatile anesthetics on phagocytes.Clinical immunology (Orlando, Fla.) · 2021Review
- TLR7 mediated viral recognition results in focal type I interferon secretion by dendritic cells.Nature communications · 2017Article
- Extracellular calcium elicits feedforward regulation of the Toll-like receptor-triggered innate immune response.Cellular & molecular immunology · 2017Article
- Distinct Effects of Integrins αXβ2 and αMβ2 on Leukocyte Subpopulations during Inflammation and Antimicrobial Responses.Infection and immunity · 2017Article
- Class I myosin Myo1e regulates TLR4-triggered macrophage spreading, chemokine release, and antigen presentation via MHC class II.European journal of immunology · 2015Article
- Antioxidative dietary compounds modulate gene expression associated with apoptosis, DNA repair, inhibition of cell proliferation and migration.International journal of molecular sciences · 2014Review
- Characterization of the effect of LPS on dendritic cell subset discrimination in spleen.Journal of cellular and molecular medicine · 2014Article
- Nitric oxide and interleukins are involved in cell proliferation of RAW264.7 macrophages activated by viili exopolysaccharides.Inflammation · 2013Article
- TLR signaling paralyzes monocyte chemotaxis through synergized effects of p38 MAPK and global Rap-1 activation.PloS one · 2012Article
- Measurement of TLR-Induced Macrophage Spreading by Automated Image Analysis: Differential Role of Myd88 and MAPK in Early and Late Responses.Frontiers in physiology · 2011Article
- Proteomic analysis of microtubule-associated proteins during macrophage activation.Molecular & cellular proteomics : MCP · 2009Article
- Oral bacteria induce a differential activation of human immunodeficiency virus-1 promoter in T cells, macrophages and dendritic cells.Oral microbiology and immunology · 2009Article
- Induction of distinct TLR2-mediated proinflammatory and proadhesive signaling pathways in response to Porphyromonas gingivalis fimbriae.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Membrane ruffles capture C3bi-opsonized particles in activated macrophages.Molecular biology of the cell · 2008Article
- Rap1a is a key regulator of fibroblast growth factor 2-induced angiogenesis and together with Rap1b controls human endothelial cell functions.Molecular and cellular biology · 2008Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Lipopolysaccharide (LPS), a component of the outer membrane of gram-negative bacteria, is a potent activator of macrophages. Besides inducing many transcriptional pathways, LPS also elicits rapid morphological changes such as cell spreading. Here we have investigated the signaling pathway that controls macrophage beta2-integrin-dependent spreading in response to LPS. We show that inhibition of the adapter protein MyD88, the interleukin-1 receptor-associated kinase Irak, the p38 mitogen-activated protein kinase, or the Ras-like GTPase Rap1 blocks LPS-induced spreading. In addition, Irak activates p38 and stimulates p38-dependent spreading. The activation of p38 by Irak requires Irak's kinase activity. We find that p38 controls spreading independently of its role in transcription but rather through activation of Rap1. Together, our results suggest that beta2-integrin-dependent spreading of macrophages in response to LPS is controlled by a linear signaling pathway via MyD88, Irak, p38, and Rap1.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.