ArticleJournal of bacteriology2001
Transcription of nhaA, the main Na(+)/H(+) antiporter of Escherichia coli, is regulated by Na(+) and growth phase.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 48 citations in OpenAlex.
- The Role of Porin Proteins and ygiU-nhaR in the Formation of Biofilm in Escherichia coli.Current microbiology · 2026Article
- The evolution of a NaJournal of bacteriology · 2026Article
- Different sodium and potassium homeostasis patterns between rare and abundant microbial taxa in biological soil crusts revealed by metatranscriptomics.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2026Article
- The evolution of a NabioRxiv : the preprint server for biology · 2026Article
- Article
- Fundamentals and Exceptions of the LysR-type Transcriptional Regulators.ACS synthetic biology · 2024Review
- Article
- RpoS and the bacterial general stress response.Microbiology and molecular biology reviews : MMBR · 2024Review
- NhaR, LeuO, and H-NS Are Part of an Expanded Regulatory Network for Ectoine Biosynthesis Expression.Applied and environmental microbiology · 2023Article
- Modulating the evolutionary trajectory of tolerance using antibiotics with different metabolic dependencies.Nature communications · 2022Article
- Divergently Transcribed ncRNAs inFrontiers in molecular biosciences · 2021Article
- Analysis of seven putative NaPhysiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Transcriptional Sequencing Uncovers Survival Mechanisms of Salmonella enterica Serovar Enteritidis in Antibacterial Egg White.mSphere · 2019Article
- Experimental Evolution of Escherichia coli K-12 at High pH and with RpoS Induction.Applied and environmental microbiology · 2018Article
- The Lysine 299 Residue Endows the Multisubunit Mrp1 Antiporter with Dominant Roles in NaApplied and environmental microbiology · 2018Article
- Article
- Multidrug resistance protein MdtM adds to the repertoire of antiporters involved in alkaline pH homeostasis in Escherichia coli.BMC microbiology · 2013Article
- Effect of overexpressing nhaA and nhaR on sodium tolerance and lactate production in Escherichia coli.Journal of biological engineering · 2013Article
- Translational repression of NhaR, a novel pathway for multi-tier regulation of biofilm circuitry by CsrA.Journal of bacteriology · 2012Article
- The critical role of S-lactoylglutathione formation during methylglyoxal detoxification in Escherichia coli.Molecular microbiology · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transcription of nhaA, encoding the main Na(+)/H(+) antiporter of Escherichia coli, is induced by Na(+), regulated by NhaR, and affected by H-NS. In this work the roles of the two nhaA promoters (P1 and P2) were studied by analysis of transcription both in vivo and in vitro and promoter mutations. We found that P1 is an NhaR-dependent, Na(+)-induced, and H-NS-affected promoter both in the exponential and stationary phases. An in vitro transcription assay demonstrated that P1 is activated by sigma(70)-RNA polymerase and both NhaR and H-NS increase the specificity of P1. Remarkably, in marked contrast to P1, P2 exhibits very low activity during the exponential phase but is induced in the stationary phase to become the major promoter. Furthermore, P2 is activated by sigma(S) and is neither induced by Na(+) nor dependent on NhaR or affected by H-NS. Hence, this work establishes that nhaA has a dual mode of regulation, each involving a different promoter, and reveals that P2 and sigma(S) together are responsible for the survival of stationary-phase cells in the presence of high Na(+), alkaline pH, and the combination of high Na(+) and alkaline pH, the most stressful condition.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.