ArticleApplied and environmental microbiology2001
Magnetic bead capture eliminates PCR inhibitors in samples collected from the airborne environment, permitting detection of Pneumocystis carinii DNA.
Article in Applied and environmental microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 60 citations in OpenAlex.
- Optimizing Lysis and Extraction Workflows for Enrichment-Free qPCR Detection ofPathogens (Basel, Switzerland) · 2026Article
- Article
- On-site airborne pathogen detection for infection risk mitigation.Chemical Society reviews · 2023Review
- Ultrasensitive hybridization capture: Reliable detection of <1 copy/mL short cell-free DNA from large-volume urine samples.PloS one · 2021Article
- A comparison of methods used to unveil the genetic and metabolic pool in the built environment.Microbiome · 2018Article
- Microbial Degradation of Forensic Samples of Biological Origin: Potential Threat to Human DNA Typing.Molecular biotechnology · 2018Review
- Release of free DNA by membrane-impaired bacterial aerosols due to aerosolization and air sampling.Applied and environmental microbiology · 2013Article
- Oligonucleotide based magnetic bead capture of Onchocerca volvulus DNA for PCR pool screening of vector black flies.PLoS neglected tropical diseases · 2012Article
- Evaluation of PCR inhibitory effect of enrichment broths and comparison of DNA extraction methods for detection of Salmonella Enteritidis using real-time PCR assay.Journal of veterinary science · 2010Article
- Pneumocystis murina colonization in immunocompetent surfactant protein A deficient mice following environmental exposure.Respiratory research · 2009Article
- High throughput screening for spores and vegetative forms of pathogenic B. anthracis by an internally controlled real-time PCR assay with automated DNA preparation.Medical microbiology and immunology · 2007Article
- Incorporating polymerase chain reaction-based identification, population characterization, and quantification of microorganisms into aerosol science: A review.Atmospheric environment (Oxford, England : 1994) · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
PCR detection methods are useful in studies of organisms not amenable to culture. Inhibitors in environmental samples can interfere with such assays. We describe a magnetic bead DNA capture protocol that removes inhibitors from outdoor air samples, maintaining the sensitivity of a 16S Pneumocystis carinii mitochondrial rRNA gene-based PCR.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.