ArticleApplied and environmental microbiology2001
Relationship between glycolysis and exopolysaccharide biosynthesis in Lactococcus lactis.
Article in Applied and environmental microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 113 citations in OpenAlex.
- Genomic and functional characterisation of exopolysaccharide biosynthesis in marine bacterium Alteromonas sp. PRIM-28 with emulsifying and anti-inflammatory activities.Antonie van Leeuwenhoek · 2026Article
- Screening and optimisation of crude exopolysaccharide production by marine Brevibacterium sp. JAB08 isolated from the jellyfish Chrysaora sp. from the coastal waters of Goa.Antonie van Leeuwenhoek · 2026Article
- Meat spoilage by bacteria: Influencing factors, volatile compounds, and organoleptic alterations.Food science of animal resources · 2026Review
- Metagenomic Insights into Microbial Community Succession and its Functional Changes during Natural Fermentation of Food Waste.Applied biochemistry and biotechnology · 2026Article
- Quantitative Proteomics Analysis Insights into the Cordyceps Polysaccharides Biosynthesis.Applied biochemistry and biotechnology · 2026Article
- Acidification by nitrogen metabolism triggers extracellular biopolymer production in an oleaginous yeast.Applied and environmental microbiology · 2025Article
- The cell surface-associated rhamnose-glucose polysaccharide represents the receptor ofApplied and environmental microbiology · 2025Article
- Microbial Dynamics on Different Microplastics in Coastal Urban Aquatic Ecosystems: The Critical Roles of Extracellular Polymeric Substances.Environmental science & technology · 2025Article
- Black Carbon Impacts onEnvironmental science & technology · 2024Article
- Glycosyltransferases Expression Changes inFoods (Basel, Switzerland) · 2023Article
- Carbon source-dependent capsule thickness regulation inFrontiers in cellular and infection microbiology · 2023Article
- Article
- Production of R- and S-1,2-propanediol in engineered Lactococcus lactis.AMB Express · 2021Article
- Application of microbial extracellular carbohydrate polymeric substances in food and allied industries.3 Biotech · 2020Review
- Assessment of exopolysaccharide production byFood science & nutrition · 2020Article
- Gross transcriptomic analysis of Pseudomonas putida for diagnosing environmental shifts.Microbial biotechnology · 2020Article
- Carbon source regulates polysaccharide capsule biosynthesis inThe Journal of biological chemistry · 2019Article
- Mechanism of Substrate Recognition and Catalysis of the Haloalkanoic Acid Dehalogenase Family Member α-Phosphoglucomutase.Biochemistry · 2018Article
- Transcriptomic Insights Into the Growth Phase- and Sugar-Associated Changes in the Exopolysaccharide Production of a High EPS-ProducingFrontiers in microbiology · 2018Article
- Interplay Between Capsule Expression and Uracil Metabolism inFrontiers in microbiology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The relationships between glucose metabolism and exopolysaccharide (EPS) production in a Lactococcus lactis strain containing the EPS gene cluster (Eps(+)) and in nonproducer strain MG5267 (Eps(-)) were characterized. The concentrations of relevant phosphorylated intermediates in EPS and cell wall biosynthetic pathways or glycolysis were determined by (31)P nuclear magnetic resonance. The concentrations of two EPS precursors, UDP-glucose and UDP-galactose, were significantly lower in the Eps(+) strain than in the Eps(-) strain. The precursors of the peptidoglycan pathway, UDP-N-acetylglucosamine and UDP-N-acetylmuramoyl-pentapeptide, were the major UDP-sugar derivatives detected in the two strains examined, but the concentration of the latter was greater in the Eps(+) strain, indicating that there is competition between EPS synthesis and cell growth. An intermediate in biosynthesis of histidine and nucleotides, 5-phosphorylribose 1-pyrophosphate, accumulated at concentrations in the millimolar range, showing that the pentose phosphate pathway was operating. Fructose 1,6-bisphosphate and glucose 6-phosphate were the prominent glycolytic intermediates during exponential growth of both strains, whereas in the stationary phase the main metabolites were 3-phosphoglyceric acid, 2-phosphoglyceric acid, and phosphoenolpyruvate. The activities of relevant enzymes, such as phosphoglucose isomerase, alpha-phosphoglucomutase, and UDP-glucose pyrophosphorylase, were identical in the two strains. (13)C enrichment on the sugar moieties of pure EPS showed that glucose 6-phosphate is the key metabolite at the branch point between glycolysis and EPS biosynthesis and ruled out involvement of the triose phosphate pool. This study provided clues for ways to enhance EPS production by genetic manipulation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.