ArticleEnvironmental health perspectives2000
Effects of dibutyl phthalate as an environmental endocrine disruptor on gonadal sex differentiation of genetic males of the frog Rana rugosa.
Article in Environmental health perspectives, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 104 citations in OpenAlex.
- Investigating Sexual Characteristics in Two Frog Species Under Exposure to River Water Polluted with Endocrine Disruptors.Animals : an open access journal from MDPI · 2025Article
- Di-butyl phthalate (DBP) induces defects during embryonic eye development in zebrafish.Ecotoxicology (London, England) · 2022Article
- Determinants of phthalate exposure among a U.S.-based group of Latino workers.International journal of hygiene and environmental health · 2021Article
- Plastics and the microbiome: impacts and solutions.Environmental microbiome · 2021Review
- Identifying sex-linked markers in Litoria aurea: a novel approach to understanding sex chromosome evolution in an amphibian.Scientific reports · 2019Article
- Di-butyl phthalate (DBP) induces craniofacial defects during embryonic development in zebrafish.Ecotoxicology (London, England) · 2019Article
- Low Dose Exposure to Di-2-Ethylhexylphthalate in Juvenile Rats Alters the Expression of Genes Related with Thyroid Hormone Regulation.Biomolecules & therapeutics · 2018Article
- Biodegradation of Di-(2-ethylhexyl) Phthalate by Rhodococcus ruber YC-YT1 in Contaminated Water and Soil.International journal of environmental research and public health · 2018Article
- Novel Functionalized Polythiophene-Coated Fe₃O₄ Nanoparticles for Magnetic Solid-Phase Extraction of Phthalates.Polymers · 2016Article
- Diverse animal models to examine potential role(s) and mechanism of endocrine disrupting chemicals on the tumor progression and prevention: Do they have tumorigenic or anti-tumorigenic property?Laboratory animal research · 2011Article
- Agricultural intensity in ovo affects growth, metamorphic development and sexual differentiation in the common toad (Bufo bufo).Ecotoxicology (London, England) · 2011Article
- A critical analysis of the biological impacts of plasticizers on wildlife.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2009Review
- Effects of Chinese domestic polychlorinated biphenyls (PCBs) on gonadal differentiation in Xenopus laevis.Environmental health perspectives · 2003Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To examine the effects of dibutyl phthalate (DBP) on gonadal sex differentiation, genetically male tadpoles of Rana rugosa were exposed to dilute solutions of DBP at concentrations of 0.1, 1, or 10 microM during days 19-23 after fertilization, which is the critical period of gonadal sex differentiation in R. rugosa. Tadpoles were necropsied on day 40. The genetically male tadpoles were produced from crossings between males (ZZ) of one local population, in which females are the heterogametic sex, and females (XX) of another local population, in which males are the heterogametic sex. As positive control groups, tadpoles were exposed to dilute solutions of 17beta-estradiol (E(2)) at concentrations of 0. 01, 0.1, or 1 microM during the same period. The internal structure of the gonads was histologically examined in a total of 30 control tadpoles, 86 E(2)-treated tadpoles, and 90 DBP-treated tadpoles. The gonads of the control tadpoles all showed the typical structure of testes. In contrast, 0.01, 0.1, and 1 microM E(2) treatments caused the undifferentiated gonads of 18, 63, and 100% of the tadpoles, respectively, to develop into gonads of complete or partial ovarian structure. After 0.1, 1, and 10 microM DBP treatment, 0, 7, and 17% of tadpoles, respectively, were similarly affected. These findings suggest that DBP was about 1,000-fold less potent than E(2). Nevertheless, DBP is an environmentally dangerous hormone that disrupts the pathways of testicular differentiation in genetically male animals.
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