ArticleJournal of muscle research and cell motility2000
Critical regulatory domains in intron 2 of a porcine sarcomeric myosin heavy chain gene.
Article in Journal of muscle research and cell motility, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Novel InDels ofAnimals : an open access journal from MDPI · 2020Article
- Association of Polymorphisms in Candidate Genes with the Litter Size in Two Sheep Breeds.Animals : an open access journal from MDPI · 2019Article
- Correlation analysis of mandarin fish (Journal of genetics · 2019Article
- Molecular analysis of the first intron in the bovine myostatin gene.Molecular biology reports · 2011Article
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1 author.
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Abstract
The porcine sarcomeric fast 2a myosin heavy chain (MyHC) gene was previously found to require a region extending 3' from the transcriptional start site for high levels of expression. Here we established the existence of two novel opposing regulatory domains in intron 2. A positive regulatory element, defined to a 75bp region, resembles a TATA-less intronic promoter, with a consensus transcription initiation element. It can up-regulate its endogenous or a heterologous muscle promoter in a position specific manner, and on its own drive a reporter gene. In tandem with it is a dominant negative regulatory element, localised to a 81bp region, which can down-regulate its native gene and a heterologous muscle promoter. Bandshift and DNase I footprinting assays demonstrated that specific nuclear factors bound to both regulatory elements are distinctly different. Both elements appear to have no counterpart in intron 2 of the porcine fast 2x and 2b MyHC genes. Taken together, we demonstrate for the first time that a 5'-end terminal intron of a sarcomeric MyHC gene contains two critical regulatory domains, which may be involved in the complexity of temporo-spatial expression.
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