ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2000
Origin sites of calcium release and calcium oscillations in frog sympathetic neurons.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Norepinephrine transporter defects lead to sympathetic hyperactivity in Familial Dysautonomia models.Nature communications · 2022Article
- Ca2+ removal by the plasma membrane Ca2+-ATPase influences the contribution of mitochondria to activity-dependent Ca2+ dynamics in Aplysia neuroendocrine cells.Journal of neurophysiology · 2016Article
- Imaging activity in astrocytes and neurons with genetically encoded calcium indicators following in utero electroporation.Frontiers in molecular neuroscience · 2015Article
- Separate Ca2+ sources are buffered by distinct Ca2+ handling systems in aplysia neuroendocrine cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Rapid elevation of calcium concentration in cultured dorsal spinal cord astrocytes by corticosterone.Neurochemical research · 2013Article
- Article
- Inhibition of ATP-induced Ca2+ influx by corticosterone in dorsal root ganglion neurons.Neurochemical research · 2010Article
- Inositol-1,4,5-trisphosphate receptor-mediated Ca2+ waves in pyramidal neuron dendrites propagate through hot spots and cold spots.The Journal of physiology · 2009Article
- Ca2+-dependent inactivation of Ca2+-induced Ca2+ release in bullfrog sympathetic neurons.The Journal of physiology · 2008Article
- Depolarization-induced calcium responses in sympathetic neurons: relative contributions from Ca2+ entry, extrusion, ER/mitochondrial Ca2+ uptake and release, and Ca2+ buffering.The Journal of general physiology · 2007Article
- Ca2+ sparks and secretion in dorsal root ganglion neurons.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Direct impact of T cells on neurons revealed by two-photon microscopy in living brain tissue.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Peripheral hot spots for local Ca2+ release after single action potentials in sympathetic ganglion neurons.Biophysical journal · 2004Article
- The plasma membrane calcium-ATPase as a major mechanism for intracellular calcium regulation in neurones from the rat superior cervical ganglion.The Journal of physiology · 2003Article
- Multiple modes of calcium-induced calcium release in sympathetic neurons II: a [Ca2+](i)- and location-dependent transition from endoplasmic reticulum Ca accumulation to net Ca release.The Journal of general physiology · 2001Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In many neurons, Ca(2+) signaling depends on efflux of Ca(2+) from intracellular stores into the cytoplasm via caffeine-sensitive ryanodine receptors (RyRs) of the endoplasmic reticulum. We have used high-speed confocal microscopy to image depolarization- and caffeine-evoked increases in cytoplasmic Ca(2+) levels in individual cultured frog sympathetic neurons. Although caffeine-evoked Ca(2+) wave fronts propagated throughout the cell, in most cells the initial Ca(2+) release was from one or more discrete sites that were several micrometers wide and located at the cell edge, even in Ca(2+)-free external solution. During cell-wide cytoplasmic [Ca(2+)] oscillations triggered by continual caffeine application, the initial Ca(2+) release that began each Ca(2+) peak was from the same subcellular site or sites. The Ca(2+) wave fronts propagated with constant amplitude; the spread was mostly via calcium-induced calcium release. Propagation was faster around the cell periphery than radially inward. Local Ca(2+) levels within the cell body could increase or decrease independently of neighboring regions, suggesting independent action of spatially separate Ca(2+) stores. Confocal imaging of fluorescent analogs of ryanodine and thapsigargin, and of MitoTracker, showed potential structural correlates to the patterns of Ca(2+) release and propagation. High densities of RyRs were found in a ring around the cell periphery, mitochondria in a broader ring just inside the RyRs, and sarco-endoplasmic reticulum Ca(2+) ATPase pumps in hot spots at the cell edge. Discrete sites at the cell edge primed to release Ca(2+) from intracellular stores might preferentially convert Ca(2+) influx through a local area of plasma membrane into a cell-wide Ca(2+) increase.
Indexed as
Identifiers
11124983PMC6773042W2163271847What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.