ArticleGenes & development2000
Mutation of a Drosophila gamma tubulin ring complex subunit encoded by discs degenerate-4 differentially disrupts centrosomal protein localization.
Article in Genes & development, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 64 citations in OpenAlex.
- A mdg4 retrotransposon screen for X-linked female sterile alleles and its relationship with the transcription factor OVO.G3 (Bethesda, Md.) · 2026Article
- AbioRxiv : the preprint server for biology · 2026Article
- Versatile gamma-tubulin complexes contribute to the dynamic organization of MTOCs during Drosophila spermatogenesis.Communications biology · 2024Article
- Review
- Article
- Microtubule Organizing Centers Contain Testis-Specific γ-TuRC Proteins in Spermatids of Drosophila.Frontiers in cell and developmental biology · 2021Article
- Tubgcp3 Is Required for Retinal Progenitor Cell Proliferation During Zebrafish Development.Frontiers in molecular neuroscience · 2019Article
- The Centrioles, Centrosomes, Basal Bodies, and Cilia ofGenetics · 2017Review
- Pericentriolar material structure and dynamics.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Review
- Differing requirements for Augmin in male meiotic and mitotic spindle formation in Drosophila.Open biology · 2014Article
- γ-Tubulin Ring Complexes and EB1 play antagonistic roles in microtubule dynamics and spindle positioning.The EMBO journal · 2014Article
- Virtual and biophysical screening targeting the γ-tubulin complex--a new target for the inhibition of microtubule nucleation.PloS one · 2013Article
- Dgp71WD is required for the assembly of the acentrosomal Meiosis I spindle, and is not a general targeting factor for the γ-TuRC.Biology open · 2012Article
- Proteomic and functional analysis of the mitotic Drosophila centrosome.The EMBO journal · 2010Article
- In vivo analysis of the functions of gamma-tubulin-complex proteins.Journal of cell science · 2009Article
- {gamma}-Tubulin ring complexes regulate microtubule plus end dynamics.The Journal of cell biology · 2009Article
- SADB phosphorylation of gamma-tubulin regulates centrosome duplication.Nature cell biology · 2009Article
- Stability of the small gamma-tubulin complex requires HCA66, a protein of the centrosome and the nucleolus.Journal of cell science · 2009Article
- gammaTub23C interacts genetically with brahma chromatin-remodeling complexes in Drosophila melanogaster.Genetics · 2008Article
- Distinct Dgrip84 isoforms correlate with distinct gamma-tubulins in Drosophila.Molecular biology of the cell · 2008Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have cloned the Drosophila gene discs degenerate-4 (dd4) and find that it encodes a component of the gamma-tubulin ring complex (gammaTuRC) homologous to Spc98 of budding yeast. This provides the first opportunity to study decreased function of a member of the gamma-tubulin ring complex, other than gamma-tubulin itself, in a metazoan cell. gamma-tubulin is no longer at the centrosomes but is dispersed throughout dd4 cells and yet bipolar metaphase spindles do form, although these have a dramatically decreased density of microtubules. Centrosomin (CNN) remains in broad discrete bodies but only at the focused poles of such spindles, whereas Asp (abnormal spindle protein) is always present at the presumptive minus ends of microtubules, whether or not they are focused. This is consistent with the proposed role of Asp in coordinating the nucleation of mitotic microtubule organizing centers. The centrosome associated protein CP190 is partially lost from the spindle poles in dd4 cells supporting a weak interaction with gamma-tubulin, and the displaced protein accumulates in the vicinity of chromosomes. Electron microscopy indicates not only that the poles of dd4 cells have irregular amounts of pericentriolar material, but also that they can have abnormal centrioles. In six dd4 cells subjected to serial sectioning centrioles were missing from one of the two poles. This suggests that in addition to its role in nucleating cytoplasmic and spindle microtubules, the gammaTuRC is also essential to the structure of centrioles and the separation of centrosomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.