ArticleProceedings of the National Academy of Sciences of the United States of America2000
Completing the heterotrimer: isolation and characterization of an Arabidopsis thaliana G protein gamma-subunit cDNA.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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82 citing papers in PubMed.
- Arabidopsis Heterotrimeric G Beta Variants Shape Plant Development and Modulate Responses to Endoplasmic Reticulum Stress and Salt Stress.Plant, cell & environment · 2026Article
- Phosphovariants of the canonical heterotrimeric Gα protein, GPA1, differentially affect G protein activity and Arabidopsis development.bioRxiv : the preprint server for biology · 2026Article
- Genome-Wide Identification of thePlants (Basel, Switzerland) · 2025Article
- Trade-Off Regulation in Plant Growth and Stress Responses Through the Role of Heterotrimeric G Protein Signaling.Plants (Basel, Switzerland) · 2024Review
- Arabidopsis AGB1 participates in salinity response through bZIP17-mediated unfolded protein response.BMC plant biology · 2024Article
- Heterotrimeric G Protein-Mediated Signaling Is Involved in Stress-Mediated Growth Inhibition inInternational journal of molecular sciences · 2023Article
- Distinctly localized lipid phosphate phosphatases mediate endoplasmic reticulum glycerolipid metabolism in Arabidopsis.The Plant cell · 2023Article
- G-Protein β-Subunit GeneInternational journal of molecular sciences · 2023Article
- The interplay of GTP-binding protein AGB1 with ER stress sensors IRE1a and IRE1b modulates Arabidopsis unfolded protein response and bacterial immunity.Plant signaling & behavior · 2022Article
- GCR1 Positively Regulates UV-B- and Ethylene-Induced Stomatal Closure via Activating GPA1-Dependent ROS and NO Production.International journal of molecular sciences · 2022Article
- Heterotrimeric G Protein Signaling in Abiotic Stress.Plants (Basel, Switzerland) · 2022Review
- Tomato and cotton G protein beta subunit mutants display constitutive autoimmune responses.Plant direct · 2021Article
- Heterotrimeric G Proteins in Plants: Canonical and Atypical Gα Subunits.International journal of molecular sciences · 2021Review
- Genome-wide survey of Gγ subunit gene family in eight Rosaceae and expression analysis of PbrGGs in pear (Pyrus bretschneideri).BMC plant biology · 2021Article
- Oligomerization of A. thaliana Heterotrimeric G Protein Subunits AGB1 and AGG2 In Vitro.The protein journal · 2020Article
- JA-Induced Endocytosis of AtRGS1 Is Involved in G-Protein Mediated JA Responses.International journal of molecular sciences · 2019Article
- TaCOLD1 defines a new regulator of plant height in bread wheat.Plant biotechnology journal · 2019Article
- Characterization of Heterotrimeric G Protein γ4 Subunit in Rice.International journal of molecular sciences · 2018Article
- Identification of Heterotrimeric G Protein γ3 Subunit in Rice Plasma Membrane.International journal of molecular sciences · 2018Article
- A kinase-dead version of FERONIA receptor-like kinase has dose-dependent impacts on rosette morphology and RALF1-mediated stomatal movements.FEBS letters · 2018Article
22 more citing papers are in PubMed but not listed here.
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Abstract
Heterotrimeric G proteins consist of three subunits (alpha, beta, and gamma). alpha- and beta- subunits have been previously cloned in plants, but the gamma-subunit has remained elusive. To isolate the gamma-subunit of a plant heterotrimeric G protein an Arabidopsis thaliana yeast two-hybrid library was screened by using a tobacco G-beta-subunit as the bait protein. One positive clone (AGG1) was isolated several times; it displays significant homology to the conserved domains of mammalian gamma-subunits. The predicted AGG1 protein sequence contains all of the typical characteristics of mammalian gamma-subunits such as small size (98 amino acids, 10.8 kDa), presence of a C-terminal CAAX box to direct isoprenyl modification, and an N-terminal alpha-helix region capable of forming a coiled-coil interaction with the beta-subunit. Northern and Southern analyses showed that AGG1 is a single-copy gene in Arabidopsis with a similar expression pattern to the Arabidopsis beta-subunit, AGB1 [Weiss, C. A., Garnaat, C. W., Mukai, K., Hu, Y. & Ma, H. (1994) Proc. Natl. Acad. Sci. USA 91, 9554-9558]. By using the yeast two-hybrid system, we show that AGG1 strongly interacts with tobacco and Arabidopsis beta-subunits. The in vivo results have been confirmed by using in vitro methods to prove the interaction between AGG1 and the Arabidopsis beta-subunit. As previously observed in mammalian systems, both the coiled-coil domain and the WD repeat regions of the beta-subunit are essential for AGG1 interaction. Also in agreement with previous observations, the removal of the N-terminal alpha-helix of the AGG1 greatly reduces but does not completely block the interaction.
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