ArticleProceedings of the National Academy of Sciences of the United States of America2000
Molecular determinants of membrane potential dependence in vertebrate gap junction channels.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 37 citations in OpenAlex.
- Skin capillary endothelial cells form a network of spatiotemporally conserved CaProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Membrane voltage multistability in coupled glial cells.bioRxiv : the preprint server for biology · 2026Article
- Skin capillary endothelial cells form a network of spatiotemporally conserved CabioRxiv : the preprint server for biology · 2026Article
- Connexins in the Heart: Regulation, Function and Involvement in Cardiac Disease.International journal of molecular sciences · 2021Review
- Exacerbation of Epilepsy by Astrocyte Alkalization and Gap Junction Uncoupling.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Calcium-Dependent Arrhythmogenic Foci Created by Weakly Coupled Myocytes in the Failing Heart.Circulation research · 2017Article
- An electrostatic mechanism for Ca(2+)-mediated regulation of gap junction channels.Nature communications · 2016Article
- Extracellular K⁺ and astrocyte signaling via connexin and pannexin channels.Neurochemical research · 2012Review
- Gap junctions.Comprehensive Physiology · 2012Review
- Biological and biophysical properties of vascular connexin channels.International review of cell and molecular biology · 2009Review
- Gap junctions: their importance for the dynamics of neural circuits.Molecular neurobiology · 2004Review
- Connexin43 orthologues in vertebrates: phylogeny from fish to man.Development genes and evolution · 2004Article
- Gap junction channel gating.Biochimica et biophysica acta · 2004Review
- Calmodulin kinase pathway mediates the K+-induced increase in Gap junctional communication between mouse spinal cord astrocytes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Gating properties of gap junction channels assembled from connexin43 and connexin43 fused with green fluorescent protein.Biophysical journal · 2001Article
- Gap junction channel gating modulated through protein phosphorylation.Progress in biophysics and molecular biologyReview
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
The conductance, g(j), of many gap junctions depends on voltage between the coupled cells (transjunctional voltage, V(j)) with little effect of the absolute membrane potential (V(m)) in the two cells; others show combined V(j) and V(m) dependence. We examined the molecular determinants of V(m) dependence by using rat connexin 43 expressed in paired Xenopus oocytes. These junctions have, in addition to V(j) dependence, V(m) dependence such that equal depolarization of both cells decreases g(j). The dependence of g(j) on V(m) was abolished by truncation of the C-terminal domain (CT) at residue 242 but not at 257. There are two charged residues between 242 and 257. In full-length Cx43, mutations neutralizing either one of these charges, Arg243Gln and Asp245Gln, decreased and increased V(m) dependence, respectively, suggesting that these residues are part of the V(m) sensor. Mutating both residues together abolished V(m) dependence, although there is no net change in charge. The neutralizing mutations, together or separately, had no effect on V(j) dependence. Thus, the voltage sensors must differ. However, V(j) gating was somewhat modulated by V(m), and V(m) gating was reduced when the V(j) gate was closed. These data suggest that the two forms of voltage dependence are mediated by separate but interacting domains.
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