ArticleProceedings of the National Academy of Sciences of the United States of America2000
Functional interaction between c-Abl and the p21-activated protein kinase gamma-PAK.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 46 citations in OpenAlex.
- Regulation of Cancer Metastasis by PAK2.International journal of molecular sciences · 2024Review
- Article
- PAK2 links cell survival to mechanotransduction and metabolism.The Journal of cell biology · 2019Article
- Synergistic Activation of ERα by Estrogen and Prolactin in Breast Cancer Cells Requires Tyrosyl Phosphorylation of PAK1.Cancer research · 2016Article
- Imatinib inhibits VEGF-independent angiogenesis by targeting neuropilin 1-dependent ABL1 activation in endothelial cells.The Journal of experimental medicine · 2014Article
- Potential compensation among group I PAK members in hindlimb ischemia and wound healing.PloS one · 2014Article
- Salmonella modulation of host cell gene expression promotes its intracellular growth.PLoS pathogens · 2013Article
- PAK signaling in cancer.Cellular logistics · 2012Article
- ABL tyrosine kinases: evolution of function, regulation, and specificity.Science signaling · 2010Review
- Pak protein kinases and their role in cancer.Cancer metastasis reviews · 2009Review
- Analysis of conformational changes during activation of protein kinase Pak2 by amide hydrogen/deuterium exchange.The Journal of biological chemistry · 2008Article
- Identification of phosphorylation sites in betaPIX and PAK1.Journal of cell science · 2007Article
- Autophosphorylation-dependent degradation of Pak1, triggered by the Rho-family GTPase, Chp.The Biochemical journal · 2007Article
- Inhibition of cap-dependent translation via phosphorylation of eIF4G by protein kinase Pak2.The EMBO journal · 2005Article
- Binding of activated alpha2-macroglobulin to its cell surface receptor GRP78 in 1-LN prostate cancer cells regulates PAK-2-dependent activation of LIMK.The Journal of biological chemistry · 2005Article
- Activation of Syk protein tyrosine kinase in response to osmotic stress requires interaction with p21-activated protein kinase Pak2/gamma-PAK.Molecular and cellular biology · 2004Article
- Cell-type-specific activation of PAK2 by transforming growth factor beta independent of Smad2 and Smad3.Molecular and cellular biology · 2003Article
- Differential interaction and activation of Rho family GTPases by p210bcr-abl and p190bcr-abl.Oncogene · 2003Article
- Cdc42/Rac1-mediated activation primes PAK2 for superactivation by tyrosine phosphorylation.Molecular and cellular biology · 2002Article
- Varicella-zoster virus ORF47 protein serine kinase: characterization of a cloned, biologically active phosphotransferase and two viral substrates, ORF62 and ORF63.Journal of virology · 2001Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
A member of the p21-activated protein kinase (PAK) family, gamma-PAK has cytostatic properties and is activated by cellular stresses such as hyperosmolarity or DNA damage. We report herein that gamma-PAK is associated in vivo with the nonreceptor protein tyrosine kinase c-Abl. gamma-PAK phosphorylates c-Abl on sites located in the kinase domain, in a region that is implicated in protein-protein interactions and in subcellular localization. Activation of gamma-PAK in human embryonic kidney 293T cells by cotransfection with constitutively active Cdc42 induces activation of c-Abl, resulting in increased phosphotyrosine levels. Cotransfection of c-Abl and gamma-PAK elicits phosphorylation of gamma-PAK on tyrosine and down-regulation of gamma-PAK activity, promoting accumulation of inactive gamma-PAK. gamma-PAK is also phosphorylated in vitro by c-Abl. gamma-PAK activity is regulated by ubiquitination and proteolysis in vivo, as shown by immunoblotting with an anti-ubiquitin antibody in the presence of proteasome inhibitors. In summary, we describe a functional interaction between gamma-PAK and c-Abl in which gamma-PAK stimulates c-Abl tyrosine kinase activity and c-Abl phosphorylates and down-regulates gamma-PAK, suggesting the existence of a negative feedback loop between c-Abl and gamma-PAK.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.