ArticleProceedings of the National Academy of Sciences of the United States of America2000
An unexpectedly high excision capacity for mispaired 5-hydroxymethyluracil in human cell extracts.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 67 citations in OpenAlex.
- 5mC and 5hmC methylation sequencing: the power of 6-base sequencing in a multiomic era.Epigenomics · 2026Review
- What is in a name? Rethinking SMUG1 in genome maintenance.NAR cancer · 2025Review
- Quantum Computational, Spectroscopic (FT-IR, FT-Raman, NMR, and UV-Vis) Hirshfeld Surface and Molecular Docking-Dynamics Studies on 5-Hydroxymethyluracil (Monomer and Trimer).Molecules (Basel, Switzerland) · 2023Article
- MeCP2 Is an Epigenetic Factor That Links DNA Methylation with Brain Metabolism.International journal of molecular sciences · 2023Review
- Chemical and enzymatic modifications of 5-methylcytosine at the intersection of DNA damage, repair, and epigenetic reprogramming.PloS one · 2022Article
- Retracted Article: Divergent synthesis of 5-substituted pyrimidine 2'-deoxynucleosides and their incorporation into oligodeoxynucleotides for the survey of uracil DNA glycosylases.Chemical science · 2020Article
- TCDD Toxicity Mediated by Epigenetic Mechanisms.International journal of molecular sciences · 2018Review
- Occurrence, Biological Consequences, and Human Health Relevance of Oxidative Stress-Induced DNA Damage.Chemical research in toxicology · 2016Review
- An interplay of the base excision repair and mismatch repair pathways in active DNA demethylation.Nucleic acids research · 2016Article
- Measurement of Postreplicative DNA Metabolism and Damage in the Rodent Brain.Chemical research in toxicology · 2015Article
- Detection of Cytosine methylation in ancient DNA from five native american populations using bisulfite sequencing.PloS one · 2015Article
- The effect of Pot1 binding on the repair of thymine analogs in a telomeric DNA sequence.Nucleic acids research · 2014Article
- Establishment of methylation patterns in ES cells.Nature structural & molecular biology · 2014Article
- 5-hydroxymethylcytosine and its potential roles in development and cancer.Epigenetics & chromatin · 2013Article
- DNA methylation dynamics in health and disease.Nature structural & molecular biology · 2013Review
- Selective capture of 5-hydroxymethylcytosine from genomic DNA.Journal of visualized experiments : JoVE · 2012Article
- Germline ablation of SMUG1 DNA glycosylase causes loss of 5-hydroxymethyluracil- and UNG-backup uracil-excision activities and increases cancer predisposition of Ung-/-Msh2-/- mice.Nucleic acids research · 2012Article
- DNA glycosylases: in DNA repair and beyond.Chromosoma · 2012Review
- DNA methyltransferase 1, cytosine methylation, and cytosine hydroxymethylation in mammalian mitochondria.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Tissue distribution of 5-hydroxymethylcytosine and search for active demethylation intermediates.PloS one · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The oxidation of thymine in DNA can generate a base pair between 5-hydroxymethyluracil (HmU) and adenine, whereas the oxidation and deamination of 5-methylcytosine (5mC) in DNA can generate a base pair between HmU and guanine. Using synthetic oligonucleotides containing HmU at a defined site, HmU-DNA glycosylase activities in HeLa cell and human fibroblast cell extracts have been observed. An HmU-DNA glycosylase activity that removes HmU mispaired with guanine has been measured. Surprisingly, the HmU:G excision activity is 60 times greater than the corresponding HmU:A activity, even though the expected rate of formation of the HmU:A base pair exceeds that of the HmU:G base pair by a factor of 10(7). The HmU:G mispair would arise from the 5mC:G base pair, and, if unrepaired, would give rise to a transition mutation. The observation of an unexpectedly high HmU:G glycosylase activity suggests that human cells may encounter the HmU:G mispair much more frequently than expected. The conversion of 5mC to HmU must be considered as a potential pathway for the generation of 5mC to T transition mutations, which are often found in human tumors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.