ArticleThe Journal of clinical investigation2000
Impaired translational response and increased protein kinase PKR expression in T cells from lupus patients.
Article in The Journal of clinical investigation, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 55 citations in OpenAlex.
- Shared signatures between rheumatoid arthritis, systemic lupus erythematosus and Sjögren's syndrome uncovered through gene expression meta-analysis.Arthritis research & therapy · 2014Pooled it
- Therapeutic application of circular RNA aptamers in a mouse model of psoriasis.Nature biotechnology · 2025Article
- Sleep-wake behavior and responses to sleep deprivation and immune challenge of protein kinase RNA-activated knockout mice.Brain, behavior, and immunity · 2024Article
- Expanding Roles of Noncoding RNAs in the Pathogenesis of Systemic Lupus Erythematosus.Current rheumatology reports · 2022Review
- Identification of polo-like kinase 1 as a therapeutic target in murine lupus.Clinical & translational immunology · 2022Article
- A tale of two proteins: PACT and PKR and their roles in inflammation.The FEBS journal · 2021Review
- Circular RNAs in Immune Response and Viral Infection.Trends in biochemical sciences · 2020Review
- Article
- Dysregulation of Signaling Pathways Due to Differentially Expressed Genes From the B-Cell Transcriptomes of Systemic Lupus Erythematosus Patients - A Bioinformatics Approach.Frontiers in bioengineering and biotechnology · 2020Article
- PKR: A Kinase to Remember.Frontiers in molecular neuroscience · 2018Review
- Recombinant human erythropoietin stimulates melanoma tumor growth through activation of initiation factor eIF4E.Oncotarget · 2017Article
- Article
- Article
- PKR mediated regulation of inflammation and IL-10 during viral encephalomyelitis.Journal of neuroimmunology · 2014Article
- The pathology of T cells in systemic lupus erythematosus.Journal of immunology research · 2014Review
- Role of PKR and Type I IFNs in viral control during primary and secondary infection.PLoS pathogens · 2010Article
- Genetic linkage of systemic lupus erythematosus to 13q32 in African American families with affected male members.Human genetics · 2005Article
- Altered regulation of IL-2 production in systemic lupus erythematosus: an evolving paradigm.The Journal of clinical investigation · 2005Article
- The regulation of protein synthesis and translation factors by CD3 and CD28 in human primary T lymphocytes.BMC biochemistry · 2002Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
Activation of peripheral blood T cells results in a rapid and substantial rise in translation rates and proliferation, but proliferation in response to mitogen stimulation is impaired in systemic lupus erythematosus (SLE). We have investigated translation rates and initiation factor activities in T cells from SLE patients in response to activating signals. Activation by PMA plus ionomycin strongly increased protein synthesis in control T cells but not in T cells from SLE patients. The rate of protein synthesis is known to be strongly dependent on the activity of two eukaryotic translation initiation factors, eIF4E and eIF2alpha. We show that following stimulation, eIF4E expression and phosphorylation increased equivalently in control and SLE T cells. Expression of eIF4E interacting proteins - eIF4G, an inducer, and 4E-BP1 and 4E-BP2, two specific repressors of eIF4E function - and the phosphorylation level of 4E-BP1, were all identical in control and SLE T cells. In contrast, the protein kinase PKR, which is responsible for the phosphorylation and consequent inhibition of eIF2alpha activity, was specifically overexpressed in activated SLE T cells, correlating with an increase in eIF2alpha phosphorylation. Therefore, high expression of PKR and subsequent eIF2alpha phosphorylation is likely responsible, at least in part, for impaired translational and proliferative responses to mitogens in T cells from SLE patients.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.