ArticleInfection and immunity2001
Synthesis and surface expression of CD14 by human endothelial cells.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 95 citations in OpenAlex.
- Extracellular Vesicles as Drivers of Lung Endothelial Dysfunction in ARDS: Mechanisms and Therapeutic Opportunities.Comprehensive Physiology · 2026Review
- The impact of copper status on lubabegron fumarate-induced growth and adipose immune cell infiltration in beef feedlot steers.Journal of animal science · 2026Article
- Understanding the development of Th2 cell-driven allergic airway disease in early life.Frontiers in allergy · 2022Review
- Tumor-associated hematopoietic stem and progenitor cells positively linked to glioblastoma progression.Nature communications · 2021Article
- Lipid Droplets Formation Represents an Integral Component of Endothelial Inflammation Induced by LPS.Cells · 2021Article
- Vascular Endothelial Cells and Innate Immunity.Arteriosclerosis, thrombosis, and vascular biology · 2020Review
- Heparanase-2 protects from LPS-mediated endothelial injury by inhibiting TLR4 signalling.Scientific reports · 2019Article
- An evolving new paradigm: endothelial cells--conditional innate immune cells.Journal of hematology & oncology · 2013Review
- CD14 contributes to warm hepatic ischemia-reperfusion injury in mice.Shock (Augusta, Ga.) · 2013Article
- AQP9 expression in glioblastoma multiforme tumors is limited to a small population of astrocytic cells and CD15(+)/CalB(+) leukocytes.PloS one · 2013Article
- Monocyte signal transduction receptors in active and latent tuberculosis.Clinical & developmental immunology · 2013Article
- Generation of functional blood vessels from a single c-kit+ adult vascular endothelial stem cell.PLoS biology · 2012Article
- Glutathione peroxidase-1 modulates lipopolysaccharide-induced adhesion molecule expression in endothelial cells by altering CD14 expression.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2010Article
- The atheroprotective properties of Hsp70: a role for Hsp70-endothelial interactions?Cell stress & chaperones · 2009Article
- TLR4 signaling is coupled to SRC family kinase activation, tyrosine phosphorylation of zonula adherens proteins, and opening of the paracellular pathway in human lung microvascular endothelia.The Journal of biological chemistry · 2008Article
- Membrane-anchored CD14 is important for induction of interleukin-8 by lipopolysaccharide and peptidoglycan in uroepithelial cells.Clinical and diagnostic laboratory immunology · 2004Article
- Regulation of endotoxin-induced proinflammatory activation in human coronary artery cells: expression of functional membrane-bound CD14 by human coronary artery smooth muscle cells.Journal of immunology (Baltimore, Md. : 1950) · 2004Article
- Porphyromonas gingivalis lipopolysaccharide antagonizes Escherichia coli lipopolysaccharide at toll-like receptor 4 in human endothelial cells.Infection and immunity · 2003Article
- Differential DNA synthesis in response to activation of protease-activated receptors on cultured guinea-pig tracheal smooth muscle cells.Naunyn-Schmiedeberg's archives of pharmacology · 2003Article
- CD14- and Toll-like receptor-dependent activation of bladder epithelial cells by lipopolysaccharide and type 1 piliated Escherichia coli.Infection and immunity · 2003Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous studies have reported that human vascular endothelial cells lack the membrane-bound lipopolysaccharide (LPS) receptor, CD14 (mCD14). By optimizing assay conditions, including the selection of anti-CD14 monoclonal antibody, we now demonstrate that human umbilical vein endothelial cells (HUVEC) express CD14 on the cell surface. Single-passage HUVEC showed approximately 20 times less expression of CD14 than monocytes. Interestingly, there was significant loss of surface CD14 expression with increasing numbers of culture passages. Evidence for synthesis of CD14 by HUVEC was provided by the finding that L-[(35)S]methionine was incorporated into CD14. In addition, the expression of CD14 on HUVEC was upregulated by LPS, lysophosphatidic acid, and tissue culture supplements, and this upregulation was dependent on protein synthesis. Furthermore, the results imply that mCD14 is required for LPS-induced activation of endothelial cells in the absence of serum and that it acts in concert with serum factors (soluble CD14). Our results provide evidence that CD14 is expressed by endothelial cells and suggest that the previous inability to observe expression of this molecule has been due to culture and staining conditions. This finding has important implications for the understanding of the mechanisms by which LPS stimulates endothelial cells and the management of sepsis caused by gram-negative bacteria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.