ArticleInfection and immunity2001
vimA gene downstream of recA is involved in virulence modulation in Porphyromonas gingivalis W83.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 59 citations in OpenAlex.
- Filifactor alocis enhances survival of Porphyromonas gingivalis W83 in response to HMolecular oral microbiology · 2024Article
- The involvement of CdhR in Porphyromonas gingivalis during nitric oxide stress.Molecular oral microbiology · 2023Article
- Complementation inJournal of bacteriology · 2021Article
- A novel gingipain regulatory gene in Porphyromonas gingivalis mediates host cell detachment and inhibition of wound closure.MicrobiologyOpen · 2020Article
- PgRsp Is a Novel Redox-Sensing Transcription Regulator Essential forMicroorganisms · 2019Article
- PGN_0297 is an essential component of the type IX secretion system (T9SS) in Porphyromonas gingivalis: Tn-seq analysis for exhaustive identification of T9SS-related genes.Microbiology and immunology · 2019Article
- Role of Acetyltransferase PG1842 in Gingipain Biogenesis in Porphyromonas gingivalis.Journal of bacteriology · 2018Article
- The Type IX Secretion System (T9SS): Highlights and Recent Insights into Its Structure and Function.Frontiers in cellular and infection microbiology · 2017Review
- A putative TetR regulator is involved in nitric oxide stress resistance in Porphyromonas gingivalis.Molecular oral microbiology · 2016Article
- The roles of RgpB and Kgp in late onset gingipain activity in the vimA-defective mutant of Porphyromonas gingivalis W83.Molecular oral microbiology · 2015Article
- Metabolome variations in the Porphyromonas gingivalis vimA mutant during hydrogen peroxide-induced oxidative stress.Molecular oral microbiology · 2015Article
- Porphyromonas gingivalis and related bacteria: from colonial pigmentation to the type IX secretion system and gliding motility.Journal of periodontal research · 2015Review
- Involvement of PG2212 zinc finger protein in the regulation of oxidative stress resistance in Porphyromonas gingivalis W83.Journal of bacteriology · 2014Article
- Sequence-independent processing site of the C-terminal domain (CTD) influences maturation of the RgpB protease from Porphyromonas gingivalis.Molecular microbiology · 2013Article
- VimA mediates multiple functions that control virulence in Porphyromonas gingivalis.Molecular oral microbiology · 2013Review
- In Porphyromonas gingivalis VimF is involved in gingipain maturation through the transfer of galactose.PloS one · 2013Article
- Protective role of the PG1036-PG1037-PG1038 operon in oxidative stress in Porphyromonas gingivalis W83.PloS one · 2013Article
- VimA-dependent modulation of the secretome in Porphyromonas gingivalis.Molecular oral microbiology · 2012Article
- Differential response of Porphyromonas gingivalis to varying levels and duration of hydrogen peroxide-induced oxidative stress.Microbiology (Reading, England) · 2012Article
- Oxidative stress resistance in Porphyromonas gingivalis.Future microbiology · 2012Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
A 0.9-kb open reading frame encoding a unique 32-kDa protein was identified downstream of the recA gene of Porphyromonas gingivalis. Reverse transcription-PCR and Northern blot analysis showed that both the recA gene and this open reading frame are part of the same transcriptional unit. This cloned fragment was insertionally inactivated using the ermF-ermAM antibiotic resistance cassette to create a defective mutant by allelic exchange. When plated on Brucella blood agar, the mutant strain, designated P. gingivalis FLL92, was non-black pigmented and showed significant reduction in beta-hemolysis compared with the parent strain, P. gingivalis W83. Arginine- and lysine-specific cysteine protease activities, which were mostly soluble, were approximately 90% lower than that of the parent strain. Expression of the rgpA, rgpB, and kgp protease genes was the same in P. gingivalis FLL92 as in the wild-type strain. In contrast to the parent strain, P. gingivalis FLL92 showed increased autoaggregration in addition to a significant reduction in hemagglutinating and hemolysin activities. In in vivo experiments using a mouse model, P. gingivalis FLL92 was dramatically less virulent than the parent strain. A molecular survey of this mutant and the parent strain using all known P. gingivalis insertion sequence elements as probes suggested that no intragenomic changes due to the movement of these elements have occurred in P. gingivalis FLL92. Taken together, these results suggest that the recA downstream gene, designated vimA (virulence-modulating gene), plays an important role in virulence modulation in P. gingivalis W83, possibly representing a novel posttranscriptional or translational regulation of virulence factors in P. gingivalis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.