ArticleInfection and immunity2001
Phenotypic analysis and virulence of Candida albicans LIG4 mutants.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
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- Emerging roles of Wss1 in the survival of Candida albicans under genotoxic stresses.Current genetics · 2021Review
- DNA damage checkpoint and repair: From the budding yeastComputational and structural biotechnology journal · 2021Review
- Use of CRISPR-Cas9 To Target Homologous Recombination Limits Transformation-Induced Genomic Changes in Candida albicans.mSphere · 2020Article
- Wss1 homolog from Candida albicans and its role in DNA-protein crosslink tolerance.Molecular microbiology · 2020Article
- Partner Choice in Spontaneous Mitotic Recombination in Wild Type and Homologous Recombination Mutants ofG3 (Bethesda, Md.) · 2019Article
- The Influence of Genetic Stability onG3 (Bethesda, Md.) · 2018Article
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- Molecular cloning, modeling and differential expression of a gene encoding a silent information regulator-like protein from Sporothrix schenckii.International journal of molecular medicine · 2014Article
- Rad52 function prevents chromosome loss and truncation in Candida albicans.Molecular microbiology · 2011Article
- Molecular and proteomic analyses highlight the importance of ubiquitination for the stress resistance, metabolic adaptation, morphogenetic regulation and virulence of Candida albicans.Molecular microbiology · 2011Article
- The Candida albicans Ku70 modulates telomere length and structure by regulating both telomerase and recombination.PloS one · 2011Article
- Role of the homologous recombination genes RAD51 and RAD59 in the resistance of Candida albicans to UV light, radiomimetic and anti-tumor compounds and oxidizing agents.Fungal genetics and biology : FG & B · 2010Article
- A single SNP, G929T (Gly310Val), determines the presence of a functional and a non-functional allele of HIS4 in Candida albicans SC5314: detection of the non-functional allele in laboratory strains.Fungal genetics and biology : FG & B · 2008Article
- Role of DNA mismatch repair and double-strand break repair in genome stability and antifungal drug resistance in Candida albicans.Eukaryotic cell · 2007Article
- Virulence and karyotype analyses of rad52 mutants of Candida albicans: regeneration of a truncated chromosome of a reintegrant strain (rad52/RAD52) in the host.Infection and immunity · 2005Article
- Ectopic expression of URA3 can influence the virulence phenotypes and proteome of Candida albicans but can be overcome by targeted reintegration of URA3 at the RPS10 locus.Eukaryotic cell · 2004Article
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
In previous studies, we reported the isolation and preliminary characterization of a DNA ligase-encoding gene of Candida albicans. This gene (LIG4) is the structural and functional homologue of both yeast and human ligase IV, which is involved in nonhomologous end joining (NHEJ) of DNA double-strand breaks. In the present study, we have shown that there are no other LIG4 homologues in C. albicans. In order to study the function of LIG4 in morphogenesis and virulence, we constructed gene deletions. LIG4 transcript levels were reduced in the heterozygote and were completely absent in null strains. Concomitantly, the heterozygote showed a pronounced defect in myceliation, which was slightly greater in the null strain. This was true with several solid and liquid media, such as Spider medium, medium 199, and 2% glucose-1% yeast extract-2% Bacto Peptone, at several pHs. Reintroduction of the wild-type allele into the null mutant partially restored the ability of cells to form hyphae. In agreement with the positive role of LIG4 in morphogenesis, we detected a significant rise in mRNA levels during the morphological transition. LIG4 is not essential for DNA replication or for the repair of DNA damage induced by ionizing radiation or UV light, indicating that these lesions are repaired primarily by homologous recombination. However, our data show that the NHEJ apparatus of C. albicans may control morphogenesis in this diploid organism. In addition, deletion of one or both copies of LIG4 resulted in attenuation of virulence in a murine model of candidiasis.
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