ArticleInfection and immunity2001
Enterotoxigenic Escherichia coli TibA glycoprotein adheres to human intestine epithelial cells.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 93 citations in OpenAlex.
- Characterization of Glycosylation-Specific Systemic and Mucosal IgA Antibody Responses toFrontiers in immunology · 2021Trial
- Novel staphylococcal glycosyltransferases SdgA and SdgB mediate immunogenicity and protection of virulence-associated cell wall proteins.PLoS pathogens · 2013Trial
- Bacterial post-translational changes: the possible enigma of multidrug resistance.Archives of microbiology · 2026Review
- Prevalence of noncanonical virulence factors in the adherence and invasion by enterotoxigenicFrontiers in cellular and infection microbiology · 2026Article
- The Role ofInternational journal of molecular sciences · 2025Review
- The Molecular Characterization of Virulence Determinants and Antibiotic Resistance Patterns in Human Bacterial Uropathogens.Antibiotics (Basel, Switzerland) · 2022Article
- Multidrug-Resistant Lineage of EnterotoxigenicFrontiers in microbiology · 2021Article
- HldE Is Important for Virulence Phenotypes in EnterotoxigenicFrontiers in cellular and infection microbiology · 2018Article
- Insights into enterotoxigenic Escherichia coli diversity in Bangladesh utilizing genomic epidemiology.Scientific reports · 2017Article
- Article
- Animal Enterotoxigenic Escherichia coli.EcoSal Plus · 2016Review
- A novel mass spectrometric strategy "BEMAP" reveals Extensive O-linked protein glycosylation in Enterotoxigenic Escherichia coli.Scientific reports · 2016Article
- TleA, a Tsh-like autotransporter identified in a human enterotoxigenic Escherichia coli strain.Infection and immunity · 2015Article
- The sweet tooth of bacteria: common themes in bacterial glycoconjugates.Microbiology and molecular biology reviews : MMBR · 2014Review
- Phenotypic and genotypic characterization of enterotoxigenic Escherichia coli clinical isolates from northern Colombia, South America.BioMed research international · 2014Article
- Recent advances in understanding enteric pathogenic Escherichia coli.Clinical microbiology reviews · 2013Review
- Article
- Global identification of prokaryotic glycoproteins based on an Escherichia coli proteome microarray.PloS one · 2012Article
- Directed evaluation of enterotoxigenic Escherichia coli autotransporter proteins as putative vaccine candidates.PLoS neglected tropical diseases · 2011Article
- Structure-function analysis of the TibA self-associating autotransporter reveals a modular organization.Infection and immunity · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enterotoxigenic Escherichia coli (ETEC) is capable of invading epithelial cell lines derived from the human ileum and colon. Two separate invasion loci (tia and tib) that direct noninvasive E. coli strains to adhere to and invade cultured human intestine epithelial cells have previously been isolated from the classical ETEC strain H10407. The tib locus directs the synthesis of TibA, a 104-kDa outer membrane glycoprotein. Synthesis of TibA is directly correlated with the adherence and invasion phenotypes of the tib locus, suggesting that this protein is an adhesin and invasin. Here we report the purification of TibA and characterization of its biological activity. TibA was purified by continuous-elution preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Purified TibA was biotin labeled and then shown to bind to HCT8 human ileocecal epithelial cells in a specific and saturable manner. Unlabeled TibA competed with biotin-labeled TibA, suggesting the presence of a specific TibA receptor in HCT8 cells. These results show that TibA acts as an adhesin. Polyclonal anti-TibA antiserum inhibited invasion of ETEC strain H10407 and of recombinant E. coli bearing tib locus clones, suggesting that TibA also acts as an invasin. The ability of TibA to direct epithelial cell adhesion suggests a role for this protein in ETEC pathogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.