ArticleThe Journal of physiology2000
Contractile properties and proteins of smooth muscles of a calponin knockout mouse.
Article in The Journal of physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 93 citations in OpenAlex.
- Mechanoregulation and function of calponin and transgelin.Biophysics reviews · 2024Review
- Evolution and function of calponin and transgelin.Frontiers in cell and developmental biology · 2023Review
- Potential prognostic and immunotherapeutic value of calponin 1: A pan-cancer analysis.Frontiers in pharmacology · 2023Article
- MYPT1 reduction is a pathogenic factor of erectile dysfunction.Communications biology · 2022Article
- Coronary Artery Spasm: Risk Factors, Pathophysiological Mechanisms and Novel Diagnostic Approaches.Reviews in cardiovascular medicine · 2022Review
- Diversification of the calponin family proteins by gene amplification and repeat expansion of calponin-like motifs.Cytoskeleton (Hoboken, N.J.) · 2021Article
- MYPT1 Down-regulation by Lipopolysaccharide-SIAH1/2 E3 Ligase-Ubiquitin-Proteasomal Degradation Contributes to Colonic Obstruction of Hirschsprung Disease.Cellular and molecular gastroenterology and hepatology · 2020Article
- Double deletion of calponin 1 and calponin 2 in mice decreases systemic blood pressure with blunted length-tension response of aortic smooth muscle.Journal of molecular and cellular cardiology · 2019Article
- Calponin-3 is critical for coordinated contractility of actin stress fibers.Scientific reports · 2018Article
- Reduced myofilament component in primary Sjögren's syndrome salivary gland myoepithelial cells.Journal of molecular histology · 2018Article
- Peripheral Airway Smooth Muscle, but Not the Trachealis, Is Hypercontractile in an Equine Model of Asthma.American journal of respiratory cell and molecular biology · 2016Article
- Mechanisms of Vascular Smooth Muscle Contraction and the Basis for Pharmacologic Treatment of Smooth Muscle Disorders.Pharmacological reviews · 2016Review
- Myoepithelial cell differentiation markers in ductal carcinoma in situ progression.The American journal of pathology · 2015Article
- UNC-87 isoforms, Caenorhabditis elegans calponin-related proteins, interact with both actin and myosin and regulate actomyosin contractility.Molecular biology of the cell · 2015Article
- A Conditional Knockout Mouse Model Reveals That Calponin-3 Is Dispensable for Early B Cell Development.PloS one · 2015Article
- Syndecan-1 regulates vascular smooth muscle cell phenotype.PloS one · 2014Article
- Unphosphorylated calponin enhances the binding force of unphosphorylated myosin to actin.Biochimica et biophysica acta · 2013Article
- Altered contractile phenotypes of intestinal smooth muscle in mice deficient in myosin phosphatase target subunit 1.Gastroenterology · 2013Article
- Alterations in the contractile phenotype of the bladder: lessons for understanding physiological and pathological remodelling of smooth muscle.Journal of cellular and molecular medicine · 2012Review
- Transient expression of myofibroblast-like cells in rat rib fracture callus.Acta orthopaedica · 2012Article
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7 authors at 3 institutions in 2 countries.
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Abstract
The role of h1-calponin in regulating the contractile properties of smooth muscle was investigated in bladder and vas deferens of mice carrying a targeted mutation in both alleles designed to inactivate the basic calponin gene. These calponin knockout (KO) mice displayed no detectable h1-calponin in their smooth muscles. The amplitudes of Ca2+ sensitization, force and Ca2+ sensitivity were not significantly different in permeabilized smooth muscle of KO compared with wild-type (WT) mice, nor were the delays in onset and half-times of Ca2+ sensitization, initiated by flash photolysis of caged GTPgammaS, different. The unloaded shortening velocity (Vus) of thiophosphorylated fibres was significantly (P<0.05) faster in the smooth muscle of KO than WT animals, but could be slowed by exogenous calponin to approximate WT levels; the concentration dependence of exogenous calponin slowing of Vus was proportional to its actomyosin binding in situ. Actin expression was reduced by 25-50%, relative to that of myosin heavy chain, in smooth muscle of KO mice, without any change in the relative distribution of the actin isoforms. We conclude that the faster Vus of smooth muscle of the KO mouse is consistent with, but does not prove without further study, physiological regulation of the crossbridge cycle by calponin. Our results show no detectable role of calponin in the signal transduction of the Ca2+-sensitization pathways in smooth muscle.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.