ArticleThe EMBO journal2000
A recurrent general RNA binding domain appended to plant methionyl-tRNA synthetase acts as a cis-acting cofactor for aminoacylation.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 72 citations in OpenAlex.
- Structural basis of tRNA recognition by the widespread OB fold.Nature communications · 2024Article
- Recognition of the tRNA structure: Everything everywhere but not all at once.Cell chemical biology · 2024Review
- Discovery of two distinct aminoacyl-tRNA synthetase complexes anchored to the Plasmodium surface tRNA import protein.The Journal of biological chemistry · 2022Article
- Article
- The Usher Syndrome Type IIIB Histidyl-tRNA Synthetase Mutation Confers Temperature Sensitivity.Biochemistry · 2017Article
- A Moonlighting Human Protein Is Involved in Mitochondrial Import of tRNA.International journal of molecular sciences · 2015Article
- Inhibition of protein synthesis and malaria parasite development by drug targeting of methionyl-tRNA synthetases.Antimicrobial agents and chemotherapy · 2015Article
- Differential expression of microRNAs in dormant bud of tea [Camellia sinensis (L.) O. Kuntze].Plant cell reports · 2014Article
- Architecture and metamorphosis.Topics in current chemistry · 2014Review
- A multiple aminoacyl-tRNA synthetase complex that enhances tRNA-aminoacylation in African trypanosomes.Molecular and cellular biology · 2013Article
- Induced tRNA import into human mitochondria: implication of a host aminoacyl-tRNA-synthetase.PloS one · 2013Article
- Article
- Dual role of methionyl-tRNA synthetase in the regulation of translation and tumor suppressor activity of aminoacyl-tRNA synthetase-interacting multifunctional protein-3.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Entamoeba lysyl-tRNA synthetase contains a cytokine-like domain with chemokine activity towards human endothelial cells.PLoS neglected tropical diseases · 2011Article
- Methionyl-tRNA synthetase from Caenorhabditis elegans: a specific multidomain organization for convergent functional evolution.Protein science : a publication of the Protein Society · 2010Article
- A tryptophan-rich peptide acts as a transcription activation domain.BMC molecular biology · 2010Article
- Functional expansion of human tRNA synthetases achieved by structural inventions.FEBS letters · 2010Review
- Evolutionary basis of converting a bacterial tRNA synthetase into a yeast cytoplasmic or mitochondrial enzyme.The Journal of biological chemistry · 2009Article
- Promoting the formation of an active synthetase/tRNA complex by a nonspecific tRNA-binding domain.The Journal of biological chemistry · 2008Article
- Article
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
Abstract
The cDNA encoding rice methionyl-tRNA synthetase was isolated. The protein exhibited a C-terminal polypeptide appended to a classical MetRS domain. This supplementary domain is related to endothelial monocyte activating polypeptide II (EMAPII), a cytokine produced in mammals after cleavage of p43, a component of the multisynthetase complex. It is also related to Arc1p and Trbp111, two tRNA binding proteins. We expressed rice MetRS and a derivative with a deletion of its EMAPII-like domain. Band-shift analysis showed that this extra-domain provides MetRS with non-specific tRNA binding properties. The EMAPII-like domain contributed a 10-fold decrease in K:(M) for tRNA in the aminoacylation reaction catalyzed by the native enzyme, as compared with the C-terminally truncated MetRS. Consequently, the EMAPII domain provides MetRS with a better catalytic efficiency at the free tRNA concentration prevailing in vivo. This domain binds the acceptor minihelix of tRNA(Met) and facilitates its aminoacylation. These results suggest that the EMAPII module could be a relic of an ancient tRNA binding domain that was incorporated into primordial synthetases for aminoacylation of RNA minihelices taken as the ancestor of modern tRNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.