ArticleThe EMBO journal2000
Sequential action of two GTPases to promote vacuole docking and fusion.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 94 citations in OpenAlex.
- The GTPase activating protein Gyp7 regulates Rab7/Ypt7 activity on late endosomes.The Journal of cell biology · 2024Article
- A two-tiered system for selective receptor and transporter protein degradation.PLoS genetics · 2022Article
- Mutant Presenilin 1 Dysregulates Exosomal Proteome Cargo Produced by Human-Induced Pluripotent Stem Cell Neurons.ACS omega · 2021Article
- A Rab prenyl membrane-anchor allows effector recognition to be regulated by guanine nucleotide.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- The Participation of Regulatory Lipids in Vacuole Homotypic Fusion.Trends in biochemical sciences · 2019Review
- The intralumenal fragment pathway mediates ESCRT-independent surface transporter down-regulation.Nature communications · 2018Article
- The NaMolecular biology of the cell · 2018Article
- How and why intralumenal membrane fragments form during vacuolar lysosome fusion.Molecular biology of the cell · 2017Article
- In vitro assay using engineered yeast vacuoles for neuronal SNARE-mediated membrane fusion.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Dynamic association of the PI3P-interacting Mon1-Ccz1 GEF with vacuoles is controlled through its phosphorylation by the type 1 casein kinase Yck3.Molecular biology of the cell · 2014Article
- Rho GTPases, phosphoinositides, and actin: a tripartite framework for efficient vesicular trafficking.Small GTPases · 2014Review
- Fusion proteins and select lipids cooperate as membrane receptors for the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) Vam7p.The Journal of biological chemistry · 2013Article
- Evolution of Tre-2/Bub2/Cdc16 (TBC) Rab GTPase-activating proteins.Molecular biology of the cell · 2013Article
- A Rab11a-Rab8a-Myo5B network promotes stretch-regulated exocytosis in bladder umbrella cells.Molecular biology of the cell · 2013Article
- Identification of genes affecting vacuole membrane fragmentation in Saccharomyces cerevisiae.PloS one · 2013Article
- Termination of isoform-selective Vps21/Rab5 signaling at endolysosomal organelles by Msb3/Gyp3.Traffic (Copenhagen, Denmark) · 2012Article
- Phosphorylation of the effector complex HOPS by the vacuolar kinase Yck3p confers Rab nucleotide specificity for vacuole docking and fusion.Molecular biology of the cell · 2012Article
- The Msb3/Gyp3 GAP controls the activity of the Rab GTPases Vps21 and Ypt7 at endosomes and vacuoles.Molecular biology of the cell · 2012Article
- Role of Rab GTPases in membrane traffic and cell physiology.Physiological reviews · 2011Review
- Rab GEFs and GAPs.Current opinion in cell biology · 2010Review
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Homotypic vacuole fusion occurs by sequential priming, docking and fusion reactions. Priming frees the HOPS complex (Vps 11, 16, 18, 33, 39 and 41) to activate Ypt7p for docking. Here we explore the roles of the GDP and GTP states of Ypt7p using Gdi1p (which extracts Ypt7:GDP), Gyp7p (a GTPase-activating protein for Ypt7p:GTP), GTPgammaS or GppNHp (non-hydrolyzable nucleotides), and mutant forms of Ypt7p that favor either GTP or GDP states. GDP-bound Ypt7p on isolated vacuoles can be extracted by Gdi1p, although only the GTP-bound state allows docking. Ypt7p is converted to the GTP-bound state after priming and stably associates with HOPS. Gyp7p can cause Ypt7p to hydrolyze bound GTP to GDP, driving HOPS release and accelerating Gdi1p-mediated release of Ypt7p. Ypt7p extraction does not inhibit the Ca(2+)-triggered cascade that leads to fusion. However, in the absence of Ypt7p, fusion is still sensitive to GTPgammaS and GppNHp, indicating that there is a second specific GTPase that regulates the calcium flux and hence fusion. Thus, two GTPases sequentially govern vacuole docking and fusion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.