ArticleThe Journal of biological chemistry2001
Polypyrimidine track-binding protein binding downstream of caspase-2 alternative exon 9 represses its inclusion.
Article in The Journal of biological chemistry, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 67 citations in OpenAlex.
- The role of alternative pre-mRNA splicing in cancer progression.Cancer cell international · 2023Review
- SRSF9 Regulates Cassette Exon Splicing of Caspase-2 by Interacting with Its Downstream Exon.Cells · 2021Article
- PTBP1 promotes the growth of breast cancer cells through the PTEN/Akt pathway and autophagy.Journal of cellular physiology · 2018Article
- High expression of PTBP1 promote invasion of colorectal cancer by alternative splicing of cortactin.Oncotarget · 2017Article
- Exon 9 skipping of apoptotic caspase-2 pre-mRNA is promoted by SRSF3 through interaction with exon 8.Biochimica et biophysica acta · 2014Article
- New insights into functional roles of the polypyrimidine tract-binding protein.International journal of molecular sciences · 2013Review
- A complex network of factors with overlapping affinities represses splicing through intronic elements.Nature structural & molecular biology · 2013Article
- Alternative pre-mRNA splicing regulation in cancer: pathways and programs unhinged.Genes & development · 2010Review
- Multiple roles for polypyrimidine tract binding (PTB) proteins in trypanosome RNA metabolism.RNA (New York, N.Y.) · 2009Article
- Up-regulation of the proapoptotic caspase 2 splicing isoform by a candidate tumor suppressor, RBM5.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Multiple and specific mRNA processing targets for the major human hnRNP proteins.Molecular and cellular biology · 2008Article
- Polypyrimidine tract-binding protein (PTB) differentially affects malignancy in a cell line-dependent manner.The Journal of biological chemistry · 2008Article
- Article
- The polypyrimidine tract binding protein (PTB) represses splicing of exon 6B from the beta-tropomyosin pre-mRNA by directly interfering with the binding of the U2AF65 subunit.Molecular and cellular biology · 2006Article
- PTB regulates the processing of a 3'-terminal exon by repressing both splicing and polyadenylation.Molecular and cellular biology · 2005Article
- Identification of sex-specific transcripts of the Anopheles gambiae doublesex gene.The Journal of experimental biology · 2005Article
- Cloning and sequence analysis of a low temperature-induced gene from trifoliate orange with unusual pre-mRNA processing.Plant cell reports · 2004Article
- The conserved RNA recognition motif 3 of U2 snRNA auxiliary factor (U2AF 65) is essential in vivo but dispensable for activity in vitro.RNA (New York, N.Y.) · 2004Article
- Expression of the splicing regulator polypyrimidine tract-binding protein in normal and neoplastic brain.Neuro-oncology · 2004Article
- Conserved sequence elements associated with exon skipping.Nucleic acids research · 2003Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have been using the caspase-2 pre-mRNA as a model system to study the importance of alternative splicing in the regulation of programmed cell death. Inclusion or skipping of a cassette-type exon in the 3' portion of this pre-mRNA leads to the production of isoforms with antagonistic activity in apoptosis. We previously identified a negative regulatory element (In100) located in the intron downstream of alternative exon 9. The upstream portion of this element harbors a decoy 3' acceptor site that engages in nonproductive commitment complex interactions with the 5' splice site of exon 9. This in turn confers a competitive advantage to the exon-skipping splicing pattern. Further characterization of the In100 element reveals a second, functionally distinct, domain located downstream from the decoy 3' acceptor site. This downstream domain harbors several polypyrimidine track-binding protein (PTB)-binding sites. We show that PTB binding to these sites correlates with the negative effect on exon 9 inclusion. Finally, we show that both domains of the In100 element can function independently to repress exon 9 inclusion, although PTB binding in the vicinity of the decoy 3' splice site can modulate its activity. Our results thus reveal a complex composite element that regulates caspase-2 exon 9 alternative splicing through a novel mechanism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.