ArticleGenome research2000
A random sequencing approach for the analysis of the Trypanosoma cruzi genome: general structure, large gene and repetitive DNA families, and gene discovery.
Article in Genome research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 59 citations in OpenAlex.
- A genome-wide analysis of genetic diversity in Trypanosoma cruzi intergenic regions.PLoS neglected tropical diseases · 2014Article
- A genomic scale map of genetic diversity in Trypanosoma cruzi.BMC genomics · 2012Article
- Article
- TcTASV: a novel protein family in trypanosoma cruzi identified from a subtractive trypomastigote cDNA library.PLoS neglected tropical diseases · 2010Article
- Genomic organization and expression profile of the mucin-associated surface protein (masp) family of the human pathogen Trypanosoma cruzi.Nucleic acids research · 2009Article
- Genomic analysis of Campylobacter fetus subspecies: identification of candidate virulence determinants and diagnostic assay targets.BMC microbiology · 2009Article
- TcSNP: a database of genetic variation in Trypanosoma cruzi.Nucleic acids research · 2009Article
- New Trypanosoma cruzi repeated element that shows site specificity for insertion.Eukaryotic cell · 2007Article
- Functional mapping of a trypanosome centromere by chromosome fragmentation identifies a 16-kb GC-rich transcriptional "strand-switch" domain as a major feature.Genome research · 2005Article
- TcruziDB: an integrated Trypanosoma cruzi genome resource.Nucleic acids research · 2004Article
- gp63 homologues in Trypanosoma cruzi: surface antigens with metalloprotease activity and a possible role in host cell infection.Infection and immunity · 2003Article
- Repetitive elements in genomes of parasitic protozoa.Microbiology and molecular biology reviews : MMBR · 2003Review
- Gene discovery in the freshwater fish parasite Trypanosoma carassii: identification of trans-sialidase-like and mucin-like genes.Infection and immunity · 2002Article
- Random sequencing of Paramecium somatic DNA.Eukaryotic cell · 2002Article
- A Trypanosoma cruzi small surface molecule provides the first immunological evidence that Chagas' disease is due to a single parasite lineage.The Journal of experimental medicine · 2002Article
- A new, expressed multigene family containing a hot spot for insertion of retroelements is associated with polymorphic subtelomeric regions of Trypanosoma brucei.Eukaryotic cell · 2002Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A random sequence survey of the genome of Trypanosoma cruzi, the agent of Chagas disease, was performed and 11,459 genomic sequences were obtained, resulting in approximately 4.3 Mb of readable sequences or approximately 10% of the parasite haploid genome. The estimated total GC content was 50.9%, with a high representation of A and T di- and trinucleotide repeats. Out of the estimated 5000 parasite genes, 947 putative new genes were identified. Another 1723 sequences corresponded to genes detected previously in T. cruzi through expression sequence tag analysis. 7735 sequences had no matches in the database, but the presence of open reading frames that passed Fickett's test suggests that some might contain coding DNA. The survey was highly redundant, with approximately 35% of the sequences included in a few large sequence families. Some of them code for protein families present in dozens of copies, including proteins essential for parasite survival and retrotransposons. Other sequence families include repetitive DNA present in thousands of copies per haploid genome. Some families in the latter group are new, parasite-specific, repetitive DNAs. These results suggest that T. cruzi could constitute an interesting model to analyze gene and genome evolution due to its plasticity in terms of sequence amplification and divergence. Additional information can be found at http://www.iib.unsam.edu.ar/tcruzi.gss. html.
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