ArticlePlant physiology2000
The ACA4 gene of Arabidopsis encodes a vacuolar membrane calcium pump that improves salt tolerance in yeast.
Article in Plant physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 85 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
85 citing papers in PubMed, 205 citations in OpenAlex.
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- Cyclic nucleotide-gated channels 14, 15, and 16 coordinate multiple signaling pathways to promote salt tolerance in rice.The Plant journal : for cell and molecular biology · 2026Article
- Chromosomal deletions in banana somaclonal variants reveal negative regulators of immunity underlyingProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Genome-Wide Characterization of thePlants (Basel, Switzerland) · 2025Article
- Transcriptome Analysis Revealed Hub Genes Related to Tipburn Resistance in Chinese Cabbage (Plants (Basel, Switzerland) · 2025Article
- Genome-Wide Identification and Expression Analysis ofInternational journal of molecular sciences · 2024Article
- Review
- Types of Membrane Transporters and the Mechanisms of Interaction between Them and Reactive Oxygen Species in Plants.Antioxidants (Basel, Switzerland) · 2024Review
- Study of red vine phenotypic plasticity across central-southern Italy sites: an integrated analysis of the transcriptome and weather indices through WGCNA.Frontiers in plant science · 2024Article
- Hypothesis paper: the development of a regulatory layer in P2B autoinhibited CaPlant signaling & behavior · 2023Article
- A Potentially Practicable Halotolerant YeastJournal of fungi (Basel, Switzerland) · 2023Article
- Yeast Heterologous Expression Systems for the Study of Plant Membrane Proteins.International journal of molecular sciences · 2023Review
- Resting cytosol Ca2+ level maintained by Ca2+ pumps affects environmental responses in Arabidopsis.Plant physiology · 2023Article
- Membrane Proteins in Plant Salinity Stress Perception, Sensing, and Response.The Journal of membrane biology · 2023Review
- The vacuolar H+/Ca transporter CAX1 participates in submergence and anoxia stress responses.Plant physiology · 2022Article
- Structure, Function, and Applications of Soybean Calcium Transporters.International journal of molecular sciences · 2022Review
- A New Discovery of Argon Functioning in Plants: Regulation of Salinity Tolerance.Antioxidants (Basel, Switzerland) · 2022Article
- Calcium channels and transporters: Roles in response to biotic and abiotic stresses.Frontiers in plant science · 2022Review
- Evolutionary and Regulatory Pattern Analysis of Soybean CaFrontiers in plant science · 2022Article
- The signatures of organellar calcium.Plant physiology · 2021Review
25 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several lines of evidence suggest that regulation of intracellular Ca(2+) levels is crucial for adaptation of plants to environmental stress. We have cloned and characterized Arabidopsis auto-inhibited Ca(2+)-ATPase, isoform 4 (ACA4), a calmodulin-regulated Ca(2+)-ATPase. Confocal laser scanning data of a green fluorescent protein-tagged version of ACA4 as well as western-blot analysis of microsomal fractions obtained from two-phase partitioning and Suc density gradient centrifugation suggest that ACA4 is localized to small vacuoles. The N terminus of ACA4 contains an auto-inhibitory domain with a binding site for calmodulin as demonstrated through calmodulin-binding studies and complementation experiments using the calcium transport yeast mutant K616. ACA4 and PMC1, the yeast vacuolar Ca(2+)-ATPase, conferred protection against osmotic stress such as high NaCl, KCl, and mannitol when expressed in the K616 strain. An N-terminally modified form of ACA4 specifically conferred increased NaCl tolerance, whereas full-length ATPase had less effect.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.