ArticlePlant physiology2000
The isolation and characterization in yeast of a gene for Arabidopsis S-adenosylmethionine:phospho-ethanolamine N-methyltransferase.
Article in Plant physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 86 citations in OpenAlex.
- A Comprehensive Functional Analysis ofPlants (Basel, Switzerland) · 2025Article
- Roles of very long-chain fatty acids in compound leaf patterning in Medicago truncatula.Plant physiology · 2023Article
- Cloning and Functional Identification of Phosphoethanolamine Methyltransferase in Soybean (Frontiers in plant science · 2021Article
- uORFs: ImportantInternational journal of molecular sciences · 2020Review
- De novo RNA sequencing analysis of Aeluropus littoralis halophyte plant under salinity stress.Scientific reports · 2020Article
- A Methyltransferase Trio Essential for Phosphatidylcholine Biosynthesis and Growth.Plant physiology · 2019Article
- Article
- Loss of PhosphoethanolaminePlant physiology · 2019Article
- Alterations in wheat pollen lipidome during high day and night temperature stress.Plant, cell & environment · 2018Article
- NMT1 and NMT3Plant physiology · 2018Article
- Pi starvation-dependent regulation of ethanolamine metabolism by phosphoethanolamine phosphatase PECP1 in Arabidopsis roots.Journal of experimental botany · 2018Article
- Cloning and Functional Analysis of Phosphoethanolamine Methyltransferase Promoter from Maize (Zea mays L.).International journal of molecular sciences · 2018Article
- Arabidopsis Serine Decarboxylase 1 (SDC1) in Phospholipid and Amino Acid Metabolism.Frontiers in plant science · 2018Article
- Evolution of the Phosphatidylcholine Biosynthesis Pathways in Green Algae: Combinatorial Diversity of Methyltransferases.Journal of molecular evolution · 2018Article
- Conformational changes in the di-domain structure ofThe Journal of biological chemistry · 2017Article
- Glycerolipid synthesis and lipid trafficking in plant mitochondria.The FEBS journal · 2017Review
- The Choline/Ethanolamine Kinase Family in Arabidopsis: Essential Role of CEK4 in Phospholipid Biosynthesis and Embryo Development.The Plant cell · 2015Article
- Role of aminoalcoholphosphotransferases 1 and 2 in phospholipid homeostasis in Arabidopsis.The Plant cell · 2015Article
- Lipid biosynthesis and protein concentration respond uniquely to phosphate supply during leaf development in highly phosphorus-efficient Hakea prostrata.Plant physiology · 2014Article
- Transcriptome profiling shows gene regulation patterns in a flavonoid pathway in response to exogenous phenylalanine in Boesenbergia rotunda cell culture.BMC genomics · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Saccharomyces cerevisiae opi3 mutant strains do not have the phospholipid N-methyltransferase that catalyzes the two terminal methylations in the phosphatidylcholine (PC) biosynthetic pathway. This results in a build up of the intermediate phosphatidylmonomethylethanolamine, causing a temperature-sensitive growth phenotype. An Arabidopsis cDNA library was used to isolate three overlapping plasmids that complemented the temperature-sensitive phenotype. Phospholipid analysis showed that the presence of the cloned cDNA caused a 65-fold reduction in the level of phosphatidylmonomethylethanolamine and a significant, though not equivalent, increase in the production of PC. Sequence analysis established that the cDNA was not homologous to OPI3 or to CHO2, the only other yeast phospholipid N-methyltransferase, but was similar to several other classes of methyltransferases. S-adenosyl-Met:phospho-base N-methyltransferase assays revealed that the cDNA catalyzed the three sequential methylations of phospho-ethanolamine to form phospho-choline. Phospho-choline is converted to PC by the CDP-choline pathway, explaining the phenotype conferred upon the yeast mutant strain by the cDNA. In accordance with this the gene has been named AtNMT1. The identification of this enzyme and the failure to isolate a plant phospholipid N-methyltransferase suggests that there are fundamental differences between the pathways utilized by yeast and by some plants for synthesis of PC.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.