ArticlePlant physiology2000
Adenosine kinase of Arabidopsis. Kinetic properties and gene expression.
Article in Plant physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 126 citations in OpenAlex.
- Genome-wide identification of ADK gene family associated with heat stress tolerance in chili (Capsicum annuum L.).BMC genomic data · 2026Article
- A monomer-dimer switch modulates the activity of plant adenosine kinase.Journal of experimental botany · 2025Article
- An epiallele of a gene encoding a PfkB-type carbohydrate kinase affects plant architecture in maize.The Plant cell · 2024Article
- Multi-omics analysis reveals genetic architecture and local adaptation of coumarins metabolites in Populus.BMC plant biology · 2024Article
- Article
- Morphological and proteomic study of waterlogging tolerance in cotton.Scientific reports · 2024Article
- A plastid nucleoside kinase is involved in inosine salvage and control of purine nucleotide biosynthesis.The Plant cell · 2023Article
- Cytokinins - regulators ofFrontiers in plant science · 2023Review
- ATP and Adenosine Metabolism in Cancer: Exploitation for Therapeutic Gain.Pharmacological reviews · 2022Review
- Mechanism of kinetin-induced death of Vicia faba ssp. minor root cortex cells.Scientific reports · 2021Article
- In vitro biosynthesis of ATP from adenosine and polyphosphate.Bioresources and bioprocessing · 2021Article
- S-Adenosyl-l-Methionine Salvage Impacts Psilocybin Formation in "Magic" Mushrooms.Chembiochem : a European journal of chemical biology · 2020Article
- Genetic dissection of heat-responsive physiological traits to improve adaptation and increase yield potential in soft winter wheat.BMC genomics · 2020Article
- A Kinase and a Glycosylase Catabolize Pseudouridine in the Peroxisome to Prevent Toxic Pseudouridine Monophosphate Accumulation.The Plant cell · 2020Article
- New Insights Into the Metabolism and Role of CytokininFrontiers in plant science · 2020Review
- Adenosine Metabolism: Emerging Concepts for Cancer Therapy.Cancer cell · 2019Review
- Transcriptome analysis of two inflorescence branching mutants reveals cytokinin is an important regulator in controlling inflorescence architecture in the woody plant Jatropha curcas.BMC plant biology · 2019Article
- Pyrimidine Salvage: Physiological Functions and Interaction with Chloroplast Biogenesis.Plant physiology · 2019Article
- Dissecting Heterosis During the Ear Inflorescence Development Stage in Maize via a Metabolomics-based Analysis.Scientific reports · 2019Article
- RNA Catabolites Contribute to the Nitrogen Pool and Support Growth Recovery of Wheat.Frontiers in plant science · 2018Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To assess the functional significance of adenosine salvage in plants, the cDNAs and genes encoding two isoforms of adenosine kinase (ADK) were isolated from Arabidopsis. The ADK1- and ADK2-coding sequences are very similar, sharing 92% and 89% amino acid and nucleotide identity, respectively. Each cDNA was overexpressed in Escherichia coli, and the catalytic activity of each isoform was determined. Both ADKs had similar catalytic properties with a K(m) and V(max)/K(m) for adenosine of 0.3 to 0.5 microM and 5.4 to 22 L min(-1) mg(-1) protein, respectively. The K(m) and V(max)/K(m) for the cytokinin riboside N(6)(isopentenyl) adenosine are 3 to 5 microM and 0.021 to 0.14 L min(-1) mg(-1) protein, respectively, suggesting that adenosine is the preferred substrate for both ADK isoforms. In Arabidopsis plants, both ADK genes are expressed constitutively, with the highest steady-state mRNA levels being found in stem and root. ADK1 transcript levels were generally higher than those of ADK2. ADK enzyme activity reflected relative ADK protein levels seen in immunoblots for leaves, flowers, and stems but only poorly so for roots, siliques, and dry seeds. The catalytic properties, tissue accumulation, and expression levels of these ADKs suggest that they play a key metabolic role in the salvage synthesis of adenylates and methyl recycling in Arabidopsis. They may also contribute to cytokinin interconversion.
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