ArticleThe Biochemical journal2001
Extracellular mechanism through the Edg family of receptors might be responsible for sphingosine-1-phosphate-induced regulation of DNA synthesis and migration of rat aortic smooth-muscle cells.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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Who cites it
20 citing papers in PubMed, 101 citations in OpenAlex.
- G Protein-Coupled Receptors at the Crossroad between Physiologic and Pathologic Angiogenesis: Old Paradigms and Emerging Concepts.International journal of molecular sciences · 2017Review
- Characterization and Expression of Sphingosine 1-Phosphate Receptors in Human and Rat Heart.Frontiers in pharmacology · 2017Article
- Exercise increases sphingoid base-1-phosphate levels in human blood and skeletal muscle in a time- and intensity-dependent manner.European journal of applied physiology · 2015Article
- Sphingosine-1-Phosphate and Its Receptors: A Mutual Link between Blood Coagulation and Inflammation.Mediators of inflammation · 2015Review
- Recruitment and retention: factors that affect pericyte migration.Cellular and molecular life sciences : CMLS · 2014Review
- Pericytes on the tumor vasculature: jekyll or hyde?Cancer microenvironment : official journal of the International Cancer Microenvironment Society · 2013Article
- Sphingosine kinase 1 in cancer.Advances in cancer research · 2013Review
- Pharmacological relevance and potential of sphingosine 1-phosphate in the vascular system.British journal of pharmacology · 2011Review
- S1P, dihydro-S1P and C24:1-ceramide levels in the HDL-containing fraction of serum inversely correlate with occurrence of ischemic heart disease.Lipids in health and disease · 2011Article
- Alkaline ceramidase 2 (ACER2) and its product dihydrosphingosine mediate the cytotoxicity of N-(4-hydroxyphenyl)retinamide in tumor cells.The Journal of biological chemistry · 2010Article
- Sphingolipids of human umbilical cord vein and their alteration in preeclampsia.Molecular and cellular biochemistry · 2010Article
- Lipids in host-pathogen interactions: pathogens exploit the complexity of the host cell lipidome.Progress in lipid research · 2010Review
- Elevated serum levels of arachidonoyl-lysophosphatidic acid and sphingosine 1-phosphate in systemic sclerosis.International journal of medical sciences · 2009Article
- Human cytomegalovirus regulates bioactive sphingolipids.The Journal of biological chemistry · 2008Article
- Sphingosine 1-phosphate differentially regulates proliferation of C2C12 reserve cells and myoblasts.Molecular and cellular biochemistry · 2008Article
- Molecular recognition in the sphingosine 1-phosphate receptor family.Journal of molecular graphics & modelling · 2008Article
- Characterization and direct quantitation of sphingoid base-1-phosphates from lipid extracts: a shotgun lipidomics approach.Journal of lipid research · 2006Article
- Sphingolipids differentially regulate mitogen-activated protein kinases and intracellular Ca2+ in vascular smooth muscle: effects on CREB activation.British journal of pharmacology · 2006Article
- Cardiovascular effects of sphingosine-1-phosphate and other sphingomyelin metabolites.British journal of pharmacology · 2004Review
- Sphingosine-1-phosphate receptor subtype-specific positive and negative regulation of Rac and haematogenous metastasis of melanoma cells.The Biochemical journal · 2003Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Exogenous sphingosine 1-phosphate (S1P) increased cytosolic Ca(2+) concentration, stimulated thymidine incorporation (DNA synthesis) and inhibited cell migration in rat aortic smooth-muscle cells (AoSMCs). Although exogenous sphingosine, a substrate of sphingosine kinase or a precursor of S1P, markedly induced the intracellular accumulation of S1P, the lipid failed to mimic the S1P-induced actions. In contrast, dihydrosphingosine 1-phosphate (DHS1P), an S1P receptor agonist, duplicated these S1P actions even though DHS1P was approx. 20-50-fold less potent than S1P. The pharmacological properties of DHS1P for the S1P receptor subtypes Edg-1, Edg-3, Edg-5 and Edg-6 were compared in Chinese hamster ovary (CHO) cells that were overexpressing the respective receptor. In these S1P-receptor-overexpressing cells, DHS1P was approx. 20-30-fold less potent than S1P for the displacement of [(3)H]S1P binding and inositol phosphate response in Edg-5-expressing CHO cells, as was the case for AoSMCs. However, it was slightly (not more than 3-fold) less potent than S1P in cells expressing Edg-1, Edg-3 or Edg-6. Of the above-mentioned four types of S1P receptor, Edg-5 was abundantly expressed in AoSMCs, as demonstrated by Northern blotting. These results suggest that the intracellular accumulation of S1P is not necessary for the S1P-induced Ca(2+) response, for the stimulation of DNA synthesis or for the inhibition of cell migration. Thus these S1P-induced actions might be mediated through extracellular (or cell-surface) S1P receptors in AoSMCs: Edg-5 might be a most important receptor subtype.
Indexed as
Identifiers
11115407PMC1221551W2036179437What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.