ArticleJournal of bacteriology2001
A long T. A tract in the upp initially transcribed region is required for regulation of upp expression by UTP-dependent reiterative transcription in Escherichia coli.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 25 citations in OpenAlex.
- Structural and mechanistic basis of reiterative transcription initiation.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Structural basis of reiterative transcription from the pyrG and pyrBI promoters by bacterial RNA polymerase.Nucleic acids research · 2020Article
- Diversity, versatility and complexity of bacterial gene regulation mechanisms: opportunities and drawbacks for applications in synthetic biology.FEMS microbiology reviews · 2019Review
- Regulation of carbamoylphosphate synthesis in Escherichia coli: an amazing metabolite at the crossroad of arginine and pyrimidine biosynthesis.Amino acids · 2018Review
- X-ray crystal structure of a reiterative transcription complex reveals an atypical RNA extension pathway.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Single nucleotide resolution RNA-seq uncovers new regulatory mechanisms in the opportunistic pathogen Streptococcus agalactiae.BMC genomics · 2015Article
- Transcription start site sequence and spacing between the -10 region and the start site affect reiterative transcription-mediated regulation of gene expression in Escherichia coli.Journal of bacteriology · 2014Article
- Regulation of gene expression by reiterative transcription.Current opinion in microbiology · 2011Review
- Regulation of pyrimidine biosynthetic gene expression in bacteria: repression without repressors.Microbiology and molecular biology reviews : MMBR · 2008Review
- RNA secondary structure regulates the translation of sxy and competence development in Haemophilus influenzae.Nucleic acids research · 2008Article
- The number of G residues in the Bacillus subtilis pyrG initially transcribed region governs reiterative transcription-mediated regulation.Journal of bacteriology · 2007Article
- Escherichia coli RNA polymerase recognition of a sigma70-dependent promoter requiring a -35 DNA element and an extended -10 TGn motif.Journal of bacteriology · 2006Article
- Vibrio cholerae ToxT independently activates the divergently transcribed aldA and tagA genes.Journal of bacteriology · 2005Article
- Transcription regulation of the EcoRV restriction-modification system.Nucleic acids research · 2005Article
- Attenuation control of pyrG expression in Bacillus subtilis is mediated by CTP-sensitive reiterative transcription.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Regulation of rRNA transcription correlates with nucleoside triphosphate sensing.Journal of bacteriology · 2001Article
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3 authors at 1 institution in 1 country.
Funding
Abstract
In Escherichia coli, pyrimidine-mediated regulation of upp expression occurs by UTP-sensitive selection of alternative transcriptional start sites, which produces transcripts that differ in the ability to be elongated. The upp initially transcribed region contains the sequence GATTTTTTTTG (nontemplate strand). Initiation can occur at either the first or the second base in this sequence (designated G6 and A7, with numbering from the promoter -10 region). High intracellular UTP levels favor initiation at position A7; however, the resulting transcripts are subject to reiterative transcription (i.e., repetitive UMP addition) within the 8-bp T. A tract in the initially transcribed region and are aborted. In contrast, low intracellular UTP levels favor initiation at position G6, which results in transcripts that can, in part, avoid reiterative transcription and be elongated normally. In this study, we examined the regulatory requirement for the long T. A tract in the upp initially transcribed region. We constructed upp promoter mutations that shorten the T. A tract to 7, 6, 5, 4, 3, or 2 bp and examined the effects of these mutations on upp expression and regulation. The results indicate that pyrimidine-mediated regulation is gradually reduced as the T. A tract is shortened from 7 to 3 bp; at which point regulation ceases. This reduction in regulation is due to large-percentage increases in upp expression in cells grown under conditions of pyrimidine excess. Quantitation of cellular transcripts and in vitro transcription studies indicate that the observed effects of a shortened T. A tract on upp expression and regulation are due to increases in the fraction of both G6- and A7-initiated transcripts that avoid reiterative transcription and are elongated normally.
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